Detailed information of TA system TA080643

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 509232..510042
• Replicon: plasmid unnamed1
• Accession: NZ_CP023246
• Organism: Bacillus cereus strain HBL-AI

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: M1QK18
• Locus tag: CJ306_RS30725
• Protein ID: WP_000787540.1
• Coordinates: 509232..509645 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: CJ306_RS30730
• Protein ID: WP_119684435.1
• Coordinates: 509635..510042 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJ306_RS30710	504486..506228	-	1743	WP_119684433.1	methyl-accepting chemotaxis protein	-
CJ306_RS30715	507209..507688	+	480	WP_088346538.1	CPBP family intramembrane metalloprotease	-
CJ306_RS30720	508406..508816	-	411	WP_119684434.1	hypothetical protein	-
CJ306_RS30725	509232..509645	-	414	WP_000787540.1	DUF86 domain-containing protein	Toxin
CJ306_RS30730	509635..510042	-	408	WP_119684435.1	nucleotidyltransferase domain-containing protein	Antitoxin
CJ306_RS30735	510412..510759	-	348	WP_119684436.1	YxeA family protein	-
CJ306_RS30740	510970..512619	-	1650	WP_119684437.1	S8 family serine peptidase	-
CJ306_RS30745	512973..513440	+	468	WP_000237075.1	RsfA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(45)	hblA / hblD / hblC	1..546185	546185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (9-99)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15474.02 Da
• Isoelectric Point: 6.2324

>T80643 WP_000787540.1 NZ_CP023246:c509645-509232 [Bacillus cereus]
MKNEVILNKISTIERCIKRIQDVYGNDPENLEDFTKQDSIILNIQRACEASIDLAMHIVAGKKLGLPQSSREAFDLLVTA
GLLSADLANKLKAMVGFRNIAVHDYQSVNLDIVRQIIEKHLTDFKLFTKEVMGILEF

Nucleotide

• Length: 414 bp

>T80643 NZ_CP023246:c509645-509232 [Bacillus cereus]
ATGAAGAATGAGGTTATTTTAAATAAAATTTCTACAATAGAGCGTTGTATAAAAAGAATCCAAGATGTGTATGGAAATGA
TCCTGAAAATTTAGAGGACTTTACAAAACAAGATTCAATAATATTGAATATACAAAGAGCTTGTGAAGCGAGTATTGATT
TAGCAATGCATATTGTTGCTGGGAAAAAATTAGGACTACCCCAATCTAGTCGAGAGGCCTTTGATTTATTGGTTACAGCA
GGATTACTTAGCGCTGATCTAGCAAATAAGTTAAAGGCGATGGTTGGATTTCGTAATATTGCTGTTCATGATTATCAAAG
TGTGAATTTAGATATTGTTCGTCAAATTATTGAAAAACATTTAACTGATTTTAAACTATTTACAAAAGAAGTAATGGGGA
TATTGGAATTTTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15755.90 Da
• Isoelectric Point: 4.3549

>AT80643 WP_119684435.1 NZ_CP023246:c510042-509635 [Bacillus cereus]
MIFQSMITDIIEKLQETVNPYWIIVFGSTAENREREDSDIDIAYVSDTTLGNYERFLLAQELANIVQRDVDLVDLSEAST
VFQAQIIHTGKTIFCSDEKRKVIFEMKTLKMYSKLNEERQVVFDDIKKRGSIYEE

Nucleotide

• Length: 408 bp

>AT80643 NZ_CP023246:c510042-509635 [Bacillus cereus]
ATGATTTTTCAAAGCATGATAACAGATATTATAGAAAAATTACAAGAGACGGTGAATCCCTATTGGATAATTGTGTTTGG
ATCAACGGCTGAGAACAGAGAACGAGAAGATAGTGATATTGATATAGCTTATGTAAGTGACACAACATTAGGGAACTATG
AACGTTTTTTATTAGCACAGGAGTTAGCTAACATTGTTCAGAGAGATGTTGATTTAGTTGATTTATCCGAAGCTTCTACA
GTTTTTCAAGCTCAGATTATACATACAGGAAAGACAATATTTTGTTCGGATGAAAAACGTAAGGTTATTTTTGAAATGAA
AACCTTAAAAATGTATTCAAAATTGAATGAAGAACGACAGGTAGTATTTGATGATATAAAGAAAAGAGGTTCTATATATG
AAGAATGA

Structures

Toxin

• AlphaFold DB: M1QK18