Detailed information of TA system TA080642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2451525..2452503
• Replicon: chromosome
• Accession: NZ_CP023245
• Organism: Bacillus cereus strain HBL-AI

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CJ306_RS12850
• Protein ID: WP_000624975.1
• Coordinates: 2451525..2452262 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: CJ306_RS12855
• Protein ID: WP_000237819.1
• Coordinates: 2452375..2452503 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJ306_RS12820	2446656..2447366	+	711	WP_000787499.1	class I SAM-dependent methyltransferase	-
CJ306_RS12825	2447484..2447891	+	408	WP_000063589.1	VOC family protein	-
CJ306_RS12830	2448225..2449007	+	783	WP_119683386.1	class I SAM-dependent methyltransferase	-
CJ306_RS12835	2449165..2449878	-	714	Protein_2411	alpha-keto acid decarboxylase family protein	-
CJ306_RS12840	2449900..2450382	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
CJ306_RS12845	2450549..2451286	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CJ306_RS12850	2451525..2452262	+	738	WP_000624975.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CJ306_RS12855	2452375..2452503	+	129	WP_000237819.1	hypothetical protein	Antitoxin
CJ306_RS12860	2452576..2452752	+	177	WP_000852629.1	stage II sporulation protein SB	-
CJ306_RS12865	2452769..2453158	-	390	WP_000713869.1	YxeA family protein	-
CJ306_RS12870	2453370..2454839	+	1470	WP_119683387.1	beta-Ala-His dipeptidase	-
CJ306_RS12875	2455085..2455894	+	810	WP_000678369.1	C39 family peptidase	-
CJ306_RS12880	2455920..2456522	+	603	WP_000517272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28278.98 Da
• Isoelectric Point: 8.2998

>T80642 WP_000624975.1 NZ_CP023245:2451525-2452262 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMAIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T80642 NZ_CP023245:2451525-2452262 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGCTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT80642 WP_000237819.1 NZ_CP023245:2452375-2452503 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT80642 NZ_CP023245:2452375-2452503 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: M1PKC2