Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 59612..60222 Replicon plasmid p65
Accession NZ_CP023210
Organism Vibrio anguillarum strain ATCC-68554

Toxin (Protein)


Gene name PumA Uniprot ID A0A3M7LLW0
Locus tag CLI14_RS19175 Protein ID WP_038529385.1
Coordinates 59612..59908 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q6W4R6
Locus tag CLI14_RS19180 Protein ID WP_011154656.1
Coordinates 59932..60222 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLI14_RS19155 54871..55785 - 915 WP_011154651.1 helicase RepA family protein -
CLI14_RS19160 55782..56807 - 1026 WP_020977927.1 hypothetical protein -
CLI14_RS19165 57727..58974 - 1248 WP_013856937.1 IS91-like element ISVa3 family transposase -
CLI14_RS19170 59253..59471 + 219 WP_107489848.1 hypothetical protein -
CLI14_RS19175 59612..59908 + 297 WP_038529385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CLI14_RS19180 59932..60222 + 291 WP_011154656.1 putative addiction module antidote protein Antitoxin
CLI14_RS19185 60351..61565 - 1215 WP_020977929.1 hypothetical protein -
CLI14_RS19190 61567..61950 - 384 WP_011154658.1 hypothetical protein -
CLI14_RS19195 61956..62927 - 972 WP_011154659.1 ParB/RepB/Spo0J family partition protein -
CLI14_RS19200 62924..63718 - 795 WP_020977930.1 ParA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bauB 1..65009 65009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11097.89 Da        Isoelectric Point: 10.7080

>T80640 WP_038529385.1 NZ_CP023210:59612-59908 [Vibrio anguillarum]
MFTIKRTQLFDDWLKTLKDAQARGAITARVQRLTQGLSGDVKPVGSGISELRIHVGKGYRVYFKRSGKQIVVLLCGGNKK
TQNQDIERAKELVQYFDL

Download         Length: 297 bp

>T80640 NZ_CP023210:59612-59908 [Vibrio anguillarum]
ATGTTCACTATAAAGCGCACGCAACTTTTTGATGATTGGTTGAAGACACTTAAAGATGCTCAGGCACGAGGTGCTATCAC
TGCTCGTGTTCAGCGCCTTACTCAGGGTTTGAGTGGGGATGTTAAGCCGGTTGGTTCGGGCATTAGTGAGCTTAGGATAC
ATGTCGGTAAAGGTTATCGAGTTTATTTTAAGCGTAGTGGAAAACAGATCGTTGTCTTACTTTGCGGTGGCAATAAGAAA
ACTCAGAATCAAGATATCGAACGCGCTAAAGAGTTGGTTCAATATTTTGATTTGTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10273.59 Da        Isoelectric Point: 4.3148

>AT80640 WP_011154656.1 NZ_CP023210:59932-60222 [Vibrio anguillarum]
MNITRLDDFDPADYLDNAESIQAYIDEAVATGDAAFISQSLGVIARAKGMNTLAEETGLSRETLYRTLSVKGNPNLKTLI
SITNALGVSVKLGSYC

Download         Length: 291 bp

>AT80640 NZ_CP023210:59932-60222 [Vibrio anguillarum]
ATGAATATTACCAGGCTCGATGATTTTGATCCTGCTGACTATCTTGATAATGCTGAATCTATACAGGCATATATTGATGA
GGCGGTAGCAACTGGTGATGCTGCTTTTATTAGTCAATCCTTGGGTGTTATAGCCCGTGCCAAAGGTATGAATACGCTAG
CAGAGGAAACCGGTTTATCTCGTGAAACTTTATATAGAACATTAAGCGTTAAAGGGAATCCTAACCTTAAAACGCTTATC
TCTATCACTAATGCGCTTGGTGTATCTGTCAAACTCGGCTCTTACTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M7LLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A3M7LK76

References