Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1073327..1073963 Replicon chromosome
Accession NZ_CP023199
Organism Bifidobacterium breve strain DRBB30

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag DRBB30_RS04890 Protein ID WP_003829234.1
Coordinates 1073327..1073683 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag DRBB30_RS04895 Protein ID WP_012577921.1
Coordinates 1073670..1073963 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRBB30_RS04860 1068518..1069429 + 912 WP_014483811.1 ABC transporter ATP-binding protein -
DRBB30_RS04865 1069626..1070252 + 627 WP_003829226.1 30S ribosomal protein S4 -
DRBB30_RS04870 1070591..1071373 - 783 WP_014483809.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
DRBB30_RS04875 1071511..1071996 - 486 WP_014483808.1 cytidine deaminase -
DRBB30_RS04880 1072087..1072443 + 357 WP_014483807.1 SdpI family protein -
DRBB30_RS04885 1072548..1073150 + 603 WP_003829233.1 transglutaminase family protein -
DRBB30_RS04890 1073327..1073683 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DRBB30_RS04895 1073670..1073963 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DRBB30_RS04900 1074114..1074278 - 165 Protein_916 IS30 family transposase -
DRBB30_RS04905 1074378..1075052 + 675 WP_015438748.1 histidine phosphatase family protein -
DRBB30_RS04910 1075135..1075539 + 405 WP_014483805.1 DUF948 domain-containing protein -
DRBB30_RS04915 1075539..1075775 + 237 WP_003829239.1 hypothetical protein -
DRBB30_RS04920 1075871..1078549 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T80628 WP_003829234.1 NZ_CP023199:c1073683-1073327 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T80628 NZ_CP023199:c1073683-1073327 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT80628 WP_012577921.1 NZ_CP023199:c1073963-1073670 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT80628 NZ_CP023199:c1073963-1073670 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References