Detailed information of TA system TA080627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 1013729..1014284
• Replicon: chromosome
• Accession: NZ_CP023199
• Organism: Bifidobacterium breve strain DRBB30

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7GSS5
• Locus tag: DRBB30_RS04600
• Protein ID: WP_003833460.1
• Coordinates: 1013729..1014052 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DRBB30_RS04605
• Protein ID: WP_106642258.1
• Coordinates: 1014039..1014284 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRBB30_RS04565	1008766..1009054	-	289	Protein_853	IS256 family transposase	-
DRBB30_RS04575	1009525..1010790	-	1266	WP_015438706.1	Fic family protein	-
DRBB30_RS04585	1011332..1011697	-	366	WP_025299744.1	YccF domain-containing protein	-
DRBB30_RS04590	1011935..1012804	+	870	WP_003829104.1	class I SAM-dependent methyltransferase	-
DRBB30_RS04595	1012898..1013512	+	615	WP_003829107.1	cupin domain-containing protein	-
DRBB30_RS04600	1013729..1014052	-	324	WP_003833460.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DRBB30_RS04605	1014039..1014284	-	246	WP_106642258.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DRBB30_RS04610	1014455..1015711	+	1257	WP_106642259.1	HipA domain-containing protein	-
DRBB30_RS04615	1015978..1016168	+	191	Protein_861	XRE family transcriptional regulator	-
DRBB30_RS04620	1016352..1017623	-	1272	WP_106642260.1	MFS transporter	-
DRBB30_RS04625	1017780..1018442	-	663	WP_019728044.1	TetR/AcrR family transcriptional regulator	-
DRBB30_RS11520	1018906..1019091	+	186	WP_003808830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11829.64 Da
• Isoelectric Point: 6.2932

>T80627 WP_003833460.1 NZ_CP023199:c1014052-1013729 [Bifidobacterium breve]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Nucleotide

• Length: 324 bp

>T80627 NZ_CP023199:c1014052-1013729 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGCT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9267.31 Da
• Isoelectric Point: 4.7719

>AT80627 WP_106642258.1 NZ_CP023199:c1014284-1014039 [Bifidobacterium breve]
MSIQIATRVSDEQAASFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT80627 NZ_CP023199:c1014284-1014039 [Bifidobacterium breve]
ATGAGCATACAGATTGCCACGCGAGTTAGTGACGAGCAGGCTGCGTCGTTTAAGGAGACGACCAGGCAGCTGGGCACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Toxin

• AlphaFold DB: A0A4R0SL31