Detailed information of TA system TA080611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 144348..145027
• Replicon: plasmid pSH1
• Accession: NZ_CP023190
• Organism: Acetobacter pomorum strain SH

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5B9GMW7
• Locus tag: CJF59_RS15170
• Protein ID: WP_058988177.1
• Coordinates: 144617..145027 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CJF59_RS15165
• Protein ID: WP_058988176.1
• Coordinates: 144348..144620 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJF59_RS15130	140043..140390	-	348	WP_116100458.1	hypothetical protein	-
CJF59_RS15135	140975..141652	+	678	WP_116100427.1	hypothetical protein	-
CJF59_RS15140	141712..142011	+	300	WP_086651094.1	hypothetical protein	-
CJF59_RS15705	142004..142150	+	147	WP_162870764.1	hypothetical protein	-
CJF59_RS15150	142874..143362	+	489	WP_116100429.1	single-stranded DNA-binding protein	-
CJF59_RS15155	143425..143862	+	438	WP_116100430.1	plasmid protein	-
CJF59_RS15160	143859..144275	+	417	WP_116100431.1	hypothetical protein	-
CJF59_RS15165	144348..144620	+	273	WP_058988176.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CJF59_RS15170	144617..145027	+	411	WP_058988177.1	type II toxin-antitoxin system VapC family toxin	Toxin
CJF59_RS15175	145096..145629	+	534	WP_116100432.1	hypothetical protein	-
CJF59_RS15180	145626..145826	+	201	WP_006560470.1	hypothetical protein	-
CJF59_RS15185	145954..146166	+	213	WP_116100433.1	hypothetical protein	-
CJF59_RS15190	146232..146411	-	180	WP_086897718.1	hypothetical protein	-
CJF59_RS15195	146418..146810	-	393	WP_116100434.1	hypothetical protein	-
CJF59_RS15200	146829..147641	-	813	WP_116100435.1	hypothetical protein	-
CJF59_RS15205	147738..148202	-	465	WP_116100436.1	hypothetical protein	-
CJF59_RS15210	148205..149053	-	849	WP_116100437.1	hypothetical protein	-
CJF59_RS15215	149171..149941	-	771	WP_116100438.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..166956	166956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14787.09 Da
• Isoelectric Point: 5.2057

>T80611 WP_058988177.1 NZ_CP023190:144617-145027 [Acetobacter pomorum]
VSAYLLDTNIISDLVRNPSGPCARRIERIDPGDLCTSIIVAAELRYGCAKKGSAKLLNRVESVLAFVPVLPLDIPADCEY
GGIRAELEAAGQTIGMNDLLIAAHAYTLKATLVTNNTREFMRIRGLKVENWLEDGT

Nucleotide

• Length: 411 bp

>T80611 NZ_CP023190:144617-145027 [Acetobacter pomorum]
GTGAGCGCCTATCTCCTTGATACCAATATCATCAGTGATCTTGTCCGAAATCCTTCCGGCCCGTGCGCGCGTCGCATCGA
ACGTATCGATCCCGGCGACCTTTGTACCAGCATTATTGTGGCCGCTGAGTTACGATATGGATGTGCTAAAAAAGGCTCTG
CAAAGCTGTTAAACCGGGTTGAAAGCGTGCTGGCGTTTGTCCCCGTGCTGCCTCTCGATATTCCTGCCGACTGTGAATAC
GGTGGCATCCGCGCCGAACTCGAAGCCGCAGGGCAGACGATCGGCATGAATGATCTGCTCATCGCGGCTCACGCCTACAC
CCTCAAGGCGACCCTGGTGACGAACAACACACGTGAGTTTATGCGCATTCGAGGCTTGAAGGTTGAAAACTGGCTGGAGG
ATGGGACGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10414.01 Da
• Isoelectric Point: 4.5749

>AT80611 WP_058988176.1 NZ_CP023190:144348-144620 [Acetobacter pomorum]
MPVPEVTPRPREVKLFRNNRSQAVRIPVEFEMPGDRVLIRRDGDKLVLEPVKTPSTLKELLMAWREEPQLAPEDDFPDIQ
DVAARPEDIL

Nucleotide

• Length: 273 bp

>AT80611 NZ_CP023190:144348-144620 [Acetobacter pomorum]
ATGCCAGTTCCTGAAGTGACTCCCCGACCGCGCGAAGTGAAACTCTTTCGGAACAATCGCAGTCAGGCTGTGCGTATTCC
AGTCGAATTTGAAATGCCAGGAGATCGCGTGCTGATACGCCGTGACGGTGACAAGCTCGTTCTGGAGCCTGTGAAAACCC
CTTCCACTCTGAAGGAGCTTCTCATGGCATGGCGCGAAGAACCGCAGCTGGCCCCAGAAGACGATTTTCCCGATATTCAG
GACGTAGCCGCCAGACCGGAAGATATTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A5B9GMW7