Detailed information of TA system TA080610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 117840..118450
• Replicon: plasmid pSH1
• Accession: NZ_CP023190
• Organism: Acetobacter pomorum strain SH

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CJF59_RS14995
• Protein ID: WP_116100408.1
• Coordinates: 117840..118253 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A252A764
• Locus tag: CJF59_RS15000
• Protein ID: WP_061498081.1
• Coordinates: 118253..118450 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJF59_RS14970	113389..114078	+	690	WP_150150560.1	hypothetical protein	-
CJF59_RS14975	114075..116132	+	2058	WP_116100404.1	ATP-dependent helicase	-
CJF59_RS14980	116368..116988	+	621	WP_162870761.1	DNA/RNA non-specific endonuclease	-
CJF59_RS14985	117299..117532	+	234	WP_116100406.1	hypothetical protein	-
CJF59_RS14995	117840..118253	-	414	WP_116100408.1	PIN domain-containing protein	Toxin
CJF59_RS15000	118253..118450	-	198	WP_061498081.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CJF59_RS15005	118648..119421	+	774	WP_150150613.1	hypothetical protein	-
CJF59_RS15010	119471..122656	-	3186	WP_116100410.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..166956	166956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-115)

Antitoxin

• SpoVT-AbrB domain (5-30)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15562.99 Da
• Isoelectric Point: 4.5934

>T80610 WP_116100408.1 NZ_CP023190:c118253-117840 [Acetobacter pomorum]
MSLFLDTNILLYAMMEEGTEKAGIADSLLLHQDCHISVQTLNEATNTLRRKTRLPLDDIKTLITDFRGLVTIHPITEAVY
SRGWAIIERYGLQTYDAMILACAMENGIETLLSEDMQNGQRIFDMITIINPFRKPAE

Nucleotide

• Length: 414 bp

>T80610 NZ_CP023190:c118253-117840 [Acetobacter pomorum]
ATGTCTCTCTTTCTGGATACGAACATCCTTCTCTATGCCATGATGGAAGAAGGAACAGAAAAGGCCGGCATTGCTGACAG
TCTCCTTCTGCATCAGGACTGCCATATCTCGGTTCAGACACTTAACGAAGCCACAAATACGCTCAGGCGTAAAACCAGAC
TTCCACTAGACGACATCAAAACATTGATTACCGACTTCCGGGGGCTGGTCACGATTCATCCCATCACGGAAGCTGTTTAC
AGCCGTGGCTGGGCCATTATCGAACGGTATGGGCTCCAGACCTATGACGCCATGATTCTGGCCTGCGCCATGGAAAATGG
CATTGAAACCCTCTTGTCCGAAGATATGCAGAACGGTCAGCGGATTTTTGACATGATAACCATCATAAACCCCTTCCGGA
AACCAGCAGAGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7393.50 Da
• Isoelectric Point: 10.4806

>AT80610 WP_061498081.1 NZ_CP023190:c118450-118253 [Acetobacter pomorum]
MQVSKWGNSLAVRIPSHIVKQLGLQEGDNVDAVFTRLKSREEALRSLKEIGKKLPSGFRFERPED

Nucleotide

• Length: 198 bp

>AT80610 NZ_CP023190:c118450-118253 [Acetobacter pomorum]
ATGCAGGTTTCCAAATGGGGTAACAGCCTTGCTGTGCGTATTCCCTCCCACATTGTCAAACAGCTTGGTTTGCAGGAAGG
CGACAATGTGGATGCTGTTTTCACACGCCTGAAATCCCGCGAAGAAGCACTCCGCTCCCTGAAAGAAATCGGGAAAAAAC
TACCTTCCGGGTTCCGTTTTGAACGTCCCGAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A252A764