Detailed information of TA system TA080608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2424876..2425431
• Replicon: chromosome
• Accession: NZ_CP023189
• Organism: Acetobacter pomorum strain SH

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: F1YWD7
• Locus tag: CJF59_RS11570
• Protein ID: WP_006117339.1
• Coordinates: 2425168..2425431 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CJF59_RS11565
• Protein ID: WP_081461476.1
• Coordinates: 2424876..2425160 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJF59_RS11535	2419922..2421514	+	1593	WP_116100314.1	hypothetical protein	-
CJF59_RS11540	2421514..2422323	+	810	WP_035354364.1	hypothetical protein	-
CJF59_RS11545	2422357..2422569	+	213	WP_003628621.1	hypothetical protein	-
CJF59_RS11550	2423156..2423614	+	459	WP_035354359.1	lysozyme	-
CJF59_RS15630	2423657..2423815	+	159	WP_162784330.1	hypothetical protein	-
CJF59_RS11555	2423880..2424572	+	693	WP_006117341.1	hypothetical protein	-
CJF59_RS11560	2424650..2424859	+	210	WP_035354356.1	hypothetical protein	-
CJF59_RS11565	2424876..2425160	-	285	WP_081461476.1	BrnA antitoxin family protein	Antitoxin
CJF59_RS11570	2425168..2425431	-	264	WP_006117339.1	BrnT family toxin	Toxin
CJF59_RS11580	2425867..2426451	-	585	WP_006116589.1	TIGR00730 family Rossman fold protein	-
CJF59_RS11585	2426500..2427732	-	1233	WP_006116590.1	MFS transporter	-
CJF59_RS11590	2427829..2428404	+	576	WP_063354804.1	NUDIX hydrolase	-
CJF59_RS11595	2428438..2428890	-	453	WP_035353112.1	protoporphyrinogen oxidase HemJ	-
CJF59_RS11600	2428893..2429594	-	702	WP_089179851.1	HAD family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-90)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10074.56 Da
• Isoelectric Point: 9.7308

>T80608 WP_006117339.1 NZ_CP023189:c2425431-2425168 [Acetobacter pomorum]
MRYDWDEAKRQSNIAKHGVDFADAEDFEWDVAFVMTSRSANEPRLVAIAPINLRLHVLVFSIETRIVRIISLRKANKREA
KLYAAQT

Nucleotide

• Length: 264 bp

>T80608 NZ_CP023189:c2425431-2425168 [Acetobacter pomorum]
ATGAGATACGATTGGGACGAAGCAAAGCGTCAAAGTAACATCGCCAAGCATGGCGTTGATTTTGCTGATGCTGAAGACTT
CGAATGGGATGTGGCTTTTGTCATGACAAGTCGTAGCGCAAACGAGCCCCGCCTTGTTGCTATTGCCCCAATCAATCTCC
GCCTGCATGTGCTGGTTTTCAGCATTGAAACACGCATTGTCCGCATCATCAGCCTACGAAAGGCCAACAAACGGGAGGCA
AAGCTTTATGCCGCGCAAACCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10284.66 Da
• Isoelectric Point: 4.7435

>AT80608 WP_081461476.1 NZ_CP023189:c2425160-2424876 [Acetobacter pomorum]
MPTDEEDALINQGILQDADNPELTDQDFLNAKPASVAVPALAAQRRVRGPQKAPTKKLVSIRLDPDLIDRLKQDGPGWQG
RVNDMLRQAIGLTS

Nucleotide

• Length: 285 bp

>AT80608 NZ_CP023189:c2425160-2424876 [Acetobacter pomorum]
ATGCCTACGGATGAAGAAGATGCCCTGATCAATCAGGGCATCTTGCAAGATGCAGATAACCCCGAGCTAACGGATCAAGA
TTTTCTCAATGCCAAACCTGCATCTGTAGCTGTGCCAGCATTAGCAGCACAGCGTAGAGTAAGAGGGCCACAAAAAGCTC
CCACAAAGAAGCTTGTATCGATTCGTCTTGACCCCGATTTGATTGACCGGCTGAAACAGGACGGCCCTGGTTGGCAAGGG
CGCGTTAACGATATGCTACGGCAAGCCATAGGCCTAACCAGTTAG

Structures

Toxin

• AlphaFold DB: A0A346DWK5