Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 95290..95815 Replicon plasmid pK518_NDM1
Accession NZ_CP023187
Organism Klebsiella michiganensis strain K518

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag CKQ55_RS31520 Protein ID WP_008322213.1
Coordinates 95290..95595 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag CKQ55_RS31525 Protein ID WP_032934863.1
Coordinates 95597..95815 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKQ55_RS31485 90391..90543 + 153 Protein_105 IS5/IS1182 family transposase -
CKQ55_RS31490 90615..91735 + 1121 WP_089046468.1 IS3 family transposase -
CKQ55_RS31495 91764..92510 + 747 Protein_107 IS5 family transposase -
CKQ55_RS31505 92672..93994 + 1323 WP_016479957.1 GntP family transporter -
CKQ55_RS31510 94193..94984 - 792 WP_008322208.1 site-specific integrase -
CKQ55_RS31515 94974..95288 - 315 WP_008322211.1 hypothetical protein -
CKQ55_RS31520 95290..95595 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
CKQ55_RS31525 95597..95815 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CKQ55_RS31530 96677..97374 - 698 Protein_113 IS1 family transposase -
CKQ55_RS31540 97587..100169 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..106579 106579
- inside IScluster/Tn - - 84266..102412 18146


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T80606 WP_008322213.1 NZ_CP023187:c95595-95290 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T80606 NZ_CP023187:c95595-95290 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT80606 WP_032934863.1 NZ_CP023187:c95815-95597 [Klebsiella michiganensis]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT80606 NZ_CP023187:c95815-95597 [Klebsiella michiganensis]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References