Detailed information of TA system TA080605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 86226..87029
• Replicon: plasmid pK518_NDM1
• Accession: NZ_CP023187
• Organism: Klebsiella michiganensis strain K518

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: W8E895
• Locus tag: CKQ55_RS31460
• Protein ID: WP_016479963.1
• Coordinates: 86226..86756 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: CKQ55_RS31465
• Protein ID: WP_022652312.1
• Coordinates: 86760..87029 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKQ55_RS31425	81441..81731	+	291	WP_004201172.1	co-chaperone GroES	-
CKQ55_RS31430	81787..83427	+	1641	WP_004201176.1	chaperonin GroEL	-
CKQ55_RS32120	83616..83792	+	177	Protein_97	IS91 family transposase	-
CKQ55_RS31440	84053..84172	+	120	Protein_98	IS30 family transposase	-
CKQ55_RS31445	84182..84931	+	750	Protein_99	IS3 family transposase	-
CKQ55_RS31450	85078..86094	-	1017	WP_087728544.1	IS5-like element IS5 family transposase	-
CKQ55_RS31460	86226..86756	-	531	WP_016479963.1	GNAT family N-acetyltransferase	Toxin
CKQ55_RS31465	86760..87029	-	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
CKQ55_RS31470	87885..88874	+	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
CKQ55_RS31475	89170..89909	-	740	Protein_104	site-specific integrase	-
CKQ55_RS31485	90391..90543	+	153	Protein_105	IS5/IS1182 family transposase	-
CKQ55_RS31490	90615..91735	+	1121	WP_089046468.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / rmtC / blaNDM-1	htpB	1..106579	106579
	-	inside	IScluster/Tn	-	-	84266..102412	18146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20350.24 Da
• Isoelectric Point: 6.4907

>T80605 WP_016479963.1 NZ_CP023187:c86756-86226 [Klebsiella michiganensis]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T80605 NZ_CP023187:c86756-86226 [Klebsiella michiganensis]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT80605 WP_022652312.1 NZ_CP023187:c87029-86760 [Klebsiella michiganensis]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT80605 NZ_CP023187:c87029-86760 [Klebsiella michiganensis]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6C0L7Q2

Antitoxin

• AlphaFold DB: A0A2Z3XDS5