Detailed information of TA system TA080604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 119588..120123
• Replicon: plasmid pK518_KPC
• Accession: NZ_CP023186
• Organism: Klebsiella michiganensis strain K518

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: CKQ55_RS30850
• Protein ID: WP_032693963.1
• Coordinates: 119836..120123 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CKQ55_RS30845
• Protein ID: WP_032693964.1
• Coordinates: 119588..119839 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKQ55_RS30810	114977..115198	-	222	WP_053390281.1	hypothetical protein	-
CKQ55_RS30815	115198..115899	-	702	WP_080884168.1	DNA methylase	-
CKQ55_RS30820	116283..116618	-	336	WP_080884169.1	hypothetical protein	-
CKQ55_RS30825	116615..116848	-	234	WP_080884170.1	hypothetical protein	-
CKQ55_RS30830	117283..117512	-	230	Protein_130	hypothetical protein	-
CKQ55_RS30835	117722..118147	+	426	WP_032693966.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
CKQ55_RS30840	118147..119421	+	1275	WP_080884171.1	Y-family DNA polymerase	-
CKQ55_RS30845	119588..119839	+	252	WP_032693964.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CKQ55_RS30850	119836..120123	+	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKQ55_RS30855	120206..120394	+	189	WP_080884172.1	hypothetical protein	-
CKQ55_RS30860	121141..121641	+	501	WP_080884173.1	hypothetical protein	-
CKQ55_RS30865	121917..122309	-	393	WP_080884174.1	plasmid partitioning/stability family protein	-
CKQ55_RS30870	122313..123287	-	975	WP_080884175.1	StbA family protein	-
CKQ55_RS30875	123527..123901	-	375	WP_088903436.1	hypothetical protein	-
CKQ55_RS30880	123901..124533	-	633	WP_080884176.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..126846	126846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T80604 WP_032693963.1 NZ_CP023186:119836-120123 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T80604 NZ_CP023186:119836-120123 [Klebsiella michiganensis]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAGGAATGGCAAAAGCTGGATAAGGCCATTCAGCAACAGTTTGC
GAAGAAGCTGAAGAAGTGCTGTGACGAACCACATATCCCCTCAGCAAAGCTGAGAGGGATAAAGGACTGCTATAAAATTA
AACTTCGCGCGTCAGGCTTTCGTCTGGTCTATCAGGTAATAGATGATCAACTGATTATTGCTGTTGTGGCTGTCGGAAAG
CGTGAACGTAGTGACGTGTACAATCTGGCCAGCGAACGGATGAGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9202.49 Da
• Isoelectric Point: 4.0785

>AT80604 WP_032693964.1 NZ_CP023186:119588-119839 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQRLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT80604 NZ_CP023186:119588-119839 [Klebsiella michiganensis]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTTAAAAAAAACCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCTATTCTGAACCGTAATCAGCCCGCTTTTTACTGCATCCCGGCAGAGCTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTTTCAGAACGCAGTAACCAGCGACTGCTTGACGTGGATCTGGAC
ACATATCTATGA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2