Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3514051..3514708 Replicon chromosome
Accession NZ_CP023185
Organism Klebsiella michiganensis strain K518

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag CKQ55_RS17445 Protein ID WP_004854060.1
Coordinates 3514298..3514708 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag CKQ55_RS17440 Protein ID WP_004124953.1
Coordinates 3514051..3514317 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKQ55_RS17425 3509285..3509710 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
CKQ55_RS17430 3509831..3512629 - 2799 WP_014839506.1 transcriptional regulator DagR -
CKQ55_RS17435 3512823..3513806 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
CKQ55_RS17440 3514051..3514317 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
CKQ55_RS17445 3514298..3514708 + 411 WP_004854060.1 protein YgfX Toxin
CKQ55_RS17450 3514717..3515238 - 522 WP_014226792.1 flavodoxin FldB -
CKQ55_RS17455 3515360..3516256 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
CKQ55_RS17460 3516279..3516992 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CKQ55_RS17465 3516998..3518731 + 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T80595 WP_004854060.1 NZ_CP023185:3514298-3514708 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T80595 NZ_CP023185:3514298-3514708 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTTGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT80595 WP_004124953.1 NZ_CP023185:3514051-3514317 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT80595 NZ_CP023185:3514051-3514317 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGATAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References