Detailed information of TA system TA080594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2695013..2695560
• Replicon: chromosome
• Accession: NZ_CP023185
• Organism: Klebsiella michiganensis strain K518

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CKQ55_RS13355
• Protein ID: WP_014839830.1
• Coordinates: 2695013..2695321 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3H0P7
• Locus tag: CKQ55_RS13360
• Protein ID: WP_014227298.1
• Coordinates: 2695324..2695560 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKQ55_RS13325	2690660..2690971	-	312	WP_014839834.1	PTS sugar transporter subunit IIB	-
CKQ55_RS13330	2691266..2692207	+	942	WP_014227303.1	LacI family transcriptional regulator	-
CKQ55_RS13335	2692231..2692524	-	294	WP_014227302.1	hypothetical protein	-
CKQ55_RS13340	2692734..2693699	+	966	WP_014839832.1	Rpn family recombination-promoting nuclease/putative transposase	-
CKQ55_RS13345	2693699..2693956	+	258	WP_038422923.1	hypothetical protein	-
CKQ55_RS13350	2693960..2694862	-	903	WP_014227300.1	EamA family transporter	-
CKQ55_RS13355	2695013..2695321	-	309	WP_014839830.1	CcdB family protein	Toxin
CKQ55_RS13360	2695324..2695560	-	237	WP_014227298.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CKQ55_RS13365	2695666..2697099	-	1434	WP_014839829.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
CKQ55_RS13370	2697124..2698026	-	903	WP_009654080.1	N-acetylmuramic acid 6-phosphate etherase	-
CKQ55_RS13375	2698187..2699353	-	1167	WP_014839828.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11724.63 Da
• Isoelectric Point: 8.8012

>T80594 WP_014839830.1 NZ_CP023185:c2695321-2695013 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGKE
VCNLNHQREVVKASMDFLFDGI

Nucleotide

• Length: 309 bp

>T80594 NZ_CP023185:c2695321-2695013 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGCCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTAAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8783.92 Da
• Isoelectric Point: 5.7928

>AT80594 WP_014227298.1 NZ_CP023185:c2695560-2695324 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT80594 NZ_CP023185:c2695560-2695324 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H0P7