Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1922374..1923178 Replicon chromosome
Accession NZ_CP023185
Organism Klebsiella michiganensis strain K518

Toxin (Protein)


Gene name yeeV Uniprot ID A0A486SQP3
Locus tag CKQ55_RS09550 Protein ID WP_040217122.1
Coordinates 1922374..1922763 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7H4M120
Locus tag CKQ55_RS09555 Protein ID WP_014837280.1
Coordinates 1922819..1923178 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKQ55_RS09520 1917416..1918210 + 795 WP_042933970.1 DNA/RNA non-specific endonuclease -
CKQ55_RS09525 1918500..1918823 + 324 WP_014837285.1 endoribonuclease SymE -
CKQ55_RS09530 1918982..1919416 + 435 WP_004098173.1 VOC family protein -
CKQ55_RS09535 1919582..1920325 + 744 WP_014837284.1 hypothetical protein -
CKQ55_RS09540 1920503..1921300 - 798 WP_014837283.1 helix-turn-helix transcriptional regulator -
CKQ55_RS09545 1921442..1922287 - 846 WP_014837282.1 DUF4942 domain-containing protein -
CKQ55_RS09550 1922374..1922763 - 390 WP_040217122.1 TA system toxin CbtA family protein Toxin
CKQ55_RS09555 1922819..1923178 - 360 WP_014837280.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CKQ55_RS09560 1923203..1923424 - 222 WP_014837279.1 DUF987 domain-containing protein -
CKQ55_RS09565 1923438..1923917 - 480 WP_014837278.1 DNA repair protein RadC -
CKQ55_RS09570 1923929..1924372 - 444 WP_014837277.1 antirestriction protein -
CKQ55_RS09575 1924452..1924700 - 249 WP_044347584.1 DUF905 domain-containing protein -
CKQ55_RS09580 1924706..1925530 - 825 WP_040217125.1 DUF945 domain-containing protein -
CKQ55_RS09585 1926070..1926543 + 474 WP_157738578.1 inovirus Gp2 family protein -
CKQ55_RS31935 1926498..1926701 + 204 WP_157738580.1 inovirus-type Gp2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1918982..1957995 39013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(3-111)

Antitoxin

Antitoxin CbeA

(16-114)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14477.57 Da        Isoelectric Point: 8.9843

>T80592 WP_040217122.1 NZ_CP023185:c1922763-1922374 [Klebsiella michiganensis]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITSIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Download         Length: 390 bp

>T80592 NZ_CP023185:c1922763-1922374 [Klebsiella michiganensis]
ATGCAAACCAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTTACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACATCCATTGATATCCTCCGGGCCAGAAAGGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGTGCTCCAGGCAAGCGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13416.24 Da        Isoelectric Point: 7.0268

>AT80592 WP_014837280.1 NZ_CP023185:c1923178-1922819 [Klebsiella michiganensis]
MSKKTTIATHDISESWWGLRRSVSACFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQSFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Download         Length: 360 bp

>AT80592 NZ_CP023185:c1923178-1922819 [Klebsiella michiganensis]
TTGTCGAAGAAAACCACGATAGCAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCGTGTTT
TGGTGCCCGACTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGCGCGAATTTTAACGGACAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486SQP3

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4M120

References