Detailed information of TA system TA080591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1866181..1866757
• Replicon: chromosome
• Accession: NZ_CP023185
• Organism: Klebsiella michiganensis strain K518

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: CKQ55_RS09285
• Protein ID: WP_042933975.1
• Coordinates: 1866470..1866757 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CKQ55_RS09280
• Protein ID: WP_014837314.1
• Coordinates: 1866181..1866483 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKQ55_RS09260	1861267..1862670	+	1404	WP_089046410.1	restriction endonuclease subunit S	-
CKQ55_RS09265	1863021..1863356	+	336	WP_014837316.1	endoribonuclease SymE	-
CKQ55_RS09270	1863809..1864720	+	912	WP_014837315.1	acetamidase/formamidase family protein	-
CKQ55_RS09275	1864717..1866060	+	1344	WP_004128506.1	APC family permease	-
CKQ55_RS09280	1866181..1866483	-	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
CKQ55_RS09285	1866470..1866757	-	288	WP_042933975.1	BrnT family toxin	Toxin
CKQ55_RS09290	1867016..1867459	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
CKQ55_RS09295	1867453..1868351	-	899	Protein_1754	LysR family transcriptional regulator	-
CKQ55_RS09300	1868439..1869221	+	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
CKQ55_RS09305	1869369..1869953	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
CKQ55_RS09310	1870099..1870899	+	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
CKQ55_RS09315	1870896..1871414	+	519	WP_014837307.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T80591 WP_042933975.1 NZ_CP023185:c1866757-1866470 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T80591 NZ_CP023185:c1866757-1866470 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT80591 WP_014837314.1 NZ_CP023185:c1866483-1866181 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT80591 NZ_CP023185:c1866483-1866181 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGGTATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2