Detailed information of TA system TA080574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1527899..1528818
• Replicon: chromosome
• Accession: NZ_CP023168
• Organism: Bacillus paralicheniformis strain 14DA11

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6I7TYV7
• Locus tag: CK945_RS07970
• Protein ID: WP_023856735.1
• Coordinates: 1528072..1528818 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A6I7TM13
• Locus tag: CK945_RS07965
• Protein ID: WP_023856734.1
• Coordinates: 1527899..1528072 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CK945_RS07945	1523324..1524421	+	1098	WP_025811596.1	mannonate dehydratase	-
CK945_RS07950	1524397..1525245	+	849	WP_023856787.1	SDR family oxidoreductase	-
CK945_RS07955	1525271..1526284	+	1014	WP_079289562.1	zinc-binding alcohol dehydrogenase family protein	-
CK945_RS07960	1526336..1527607	+	1272	WP_096748019.1	MFS transporter	-
CK945_RS07965	1527899..1528072	-	174	WP_023856734.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CK945_RS07970	1528072..1528818	-	747	WP_023856735.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CK945_RS07975	1528929..1529927	-	999	WP_075212749.1	inorganic phosphate transporter	-
CK945_RS07980	1529940..1530557	-	618	WP_023856737.1	DUF47 domain-containing protein	-
CK945_RS07985	1530889..1532646	+	1758	WP_025811589.1	gamma-glutamyltransferase	-
CK945_RS07990	1532781..1533425	+	645	WP_003180888.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29421.87 Da
• Isoelectric Point: 4.6015

>T80574 WP_023856735.1 NZ_CP023168:c1528818-1528072 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEDGDD

Nucleotide

• Length: 747 bp

>T80574 NZ_CP023168:c1528818-1528072 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACGTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCTTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGATTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6897.99 Da
• Isoelectric Point: 10.8356

>AT80574 WP_023856734.1 NZ_CP023168:c1528072-1527899 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKKQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT80574 NZ_CP023168:c1528072-1527899 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6I7TYV7

Antitoxin

• AlphaFold DB: A0A6I7TM13