Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3592278..3593076 | Replicon | chromosome |
| Accession | NZ_CP023165 | ||
| Organism | Escherichia coli O22:H8 strain RM10809-3 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A1M0ZYM8 |
| Locus tag | CLC88_RS18315 | Protein ID | WP_000854904.1 |
| Coordinates | 3592699..3593076 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A093DMQ4 |
| Locus tag | CLC88_RS18310 | Protein ID | WP_001280952.1 |
| Coordinates | 3592278..3592652 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CLC88_RS18270 | 3587701..3588606 | + | 906 | Protein_3447 | IS3 family transposase | - |
| CLC88_RS18275 | 3588617..3589051 | + | 435 | Protein_3448 | hypothetical protein | - |
| CLC88_RS18280 | 3589127..3589582 | + | 456 | Protein_3449 | hypothetical protein | - |
| CLC88_RS18285 | 3589661..3589894 | + | 234 | WP_001119729.1 | DUF905 family protein | - |
| CLC88_RS18290 | 3589981..3590799 | + | 819 | WP_001234620.1 | DUF945 domain-containing protein | - |
| CLC88_RS18295 | 3590854..3591339 | + | 486 | WP_000849591.1 | antirestriction protein | - |
| CLC88_RS18300 | 3591355..3591831 | + | 477 | WP_001186726.1 | RadC family protein | - |
| CLC88_RS18305 | 3591894..3592115 | + | 222 | WP_050864148.1 | DUF987 domain-containing protein | - |
| CLC88_RS18310 | 3592278..3592652 | + | 375 | WP_001280952.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| CLC88_RS18315 | 3592699..3593076 | + | 378 | WP_000854904.1 | TA system toxin CbtA family protein | Toxin |
| CLC88_RS18320 | 3593073..3593561 | + | 489 | WP_000779174.1 | hypothetical protein | - |
| CLC88_RS18325 | 3593573..3593770 | + | 198 | WP_000839249.1 | DUF957 domain-containing protein | - |
| CLC88_RS18330 | 3593855..3594697 | + | 843 | Protein_3459 | DUF4942 domain-containing protein | - |
| CLC88_RS18335 | 3595040..3595576 | - | 537 | WP_000942786.1 | GspM family type II secretion system protein YghD | - |
| CLC88_RS18340 | 3595578..3596756 | - | 1179 | WP_155705057.1 | type II secretion system protein GspL | - |
| CLC88_RS18345 | 3596753..3597730 | - | 978 | WP_000633238.1 | type II secretion system minor pseudopilin GspK | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | espP / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC | 3514536..3654607 | 140071 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14280.26 Da Isoelectric Point: 7.2922
>T80568 WP_000854904.1 NZ_CP023165:3592699-3593076 [Escherichia coli O22:H8]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ
Download Length: 378 bp
>T80568 NZ_CP023165:3592699-3593076 [Escherichia coli O22:H8]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13719.50 Da Isoelectric Point: 6.6249
>AT80568 WP_001280952.1 NZ_CP023165:3592278-3592652 [Escherichia coli O22:H8]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS
Download Length: 375 bp
>AT80568 NZ_CP023165:3592278-3592652 [Escherichia coli O22:H8]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1M0ZYM8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A093DMQ4 |