Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3286989..3287572 Replicon chromosome
Accession NZ_CP023165
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name mazF Uniprot ID F4NJF2
Locus tag CLC88_RS16880 Protein ID WP_000254731.1
Coordinates 3286989..3287324 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag CLC88_RS16885 Protein ID WP_000581937.1
Coordinates 3287324..3287572 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS16860 3282458..3282820 + 363 WP_000034929.1 hypothetical protein -
CLC88_RS16865 3282876..3284174 - 1299 WP_000036723.1 phosphopyruvate hydratase -
CLC88_RS16870 3284262..3285899 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CLC88_RS16875 3286127..3286918 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
CLC88_RS16880 3286989..3287324 - 336 WP_000254731.1 endoribonuclease MazF Toxin
CLC88_RS16885 3287324..3287572 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CLC88_RS16890 3287650..3289884 - 2235 WP_000226815.1 GTP pyrophosphokinase -
CLC88_RS16895 3289932..3291233 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12043.02 Da        Isoelectric Point: 8.1170

>T80566 WP_000254731.1 NZ_CP023165:c3287324-3286989 [Escherichia coli O22:H8]
MVSRYVPDMGDLICVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T80566 NZ_CP023165:c3287324-3286989 [Escherichia coli O22:H8]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGTGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGTCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT80566 WP_000581937.1 NZ_CP023165:c3287572-3287324 [Escherichia coli O22:H8]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT80566 NZ_CP023165:c3287572-3287324 [Escherichia coli O22:H8]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F4NJF2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References