Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3153705..3154537 Replicon chromosome
Accession NZ_CP023165
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2A2C6E0
Locus tag CLC88_RS16180 Protein ID WP_000854716.1
Coordinates 3154163..3154537 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3U2KGT7
Locus tag CLC88_RS16175 Protein ID WP_001285595.1
Coordinates 3153705..3154073 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS16155 3151458..3152276 + 819 WP_033801547.1 DUF945 domain-containing protein -
CLC88_RS16160 3152368..3152853 + 486 WP_000213718.1 antirestriction protein -
CLC88_RS16165 3152869..3153345 + 477 WP_001379208.1 RadC family protein -
CLC88_RS16170 3153408..3153647 + 240 WP_000692304.1 DUF987 domain-containing protein -
CLC88_RS16175 3153705..3154073 + 369 WP_001285595.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CLC88_RS16180 3154163..3154537 + 375 WP_000854716.1 TA system toxin CbtA family protein Toxin
CLC88_RS16185 3154534..3155025 + 492 WP_000976843.1 hypothetical protein -
CLC88_RS16190 3155037..3155234 + 198 WP_000839249.1 DUF957 domain-containing protein -
CLC88_RS16195 3155319..3156164 + 846 WP_001280505.1 DUF4942 domain-containing protein -
CLC88_RS25745 3156899..3159152 + 2254 Protein_3047 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13986.02 Da        Isoelectric Point: 7.2426

>T80565 WP_000854716.1 NZ_CP023165:3154163-3154537 [Escherichia coli O22:H8]
MKTLPDTHVREASRCPSPVIIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYLEAK

Download         Length: 375 bp

>T80565 NZ_CP023165:3154163-3154537 [Escherichia coli O22:H8]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGATTCTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAATTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCTGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13494.33 Da        Isoelectric Point: 6.3132

>AT80565 WP_001285595.1 NZ_CP023165:3153705-3154073 [Escherichia coli O22:H8]
VSDTLPGTTLPDDNHDRPWWGLPCTVTSCFGARLVQVGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT80565 NZ_CP023165:3153705-3154073 [Escherichia coli O22:H8]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GTCCTGTTTCGGCGCCCGTCTGGTGCAGGTGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGTTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A2C6E0

Antitoxin

Source ID Structure
AlphaFold DB A0A3U2KGT7

References