Detailed information of TA system TA080551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1337400..1337879
• Replicon: chromosome
• Accession: NZ_CP023165
• Organism: Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A234Y3J1
• Locus tag: CLC88_RS07030
• Protein ID: WP_001444087.1
• Coordinates: 1337400..1337687 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A234Y267
• Locus tag: CLC88_RS07035
• Protein ID: WP_001090455.1
• Coordinates: 1337691..1337879 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLC88_RS06995	1332612..1332881	-	270	WP_000003742.1	excisionase	-
CLC88_RS07000	1332943..1335345	-	2403	WP_022581375.1	exonuclease	-
CLC88_RS07005	1335438..1335626	-	189	WP_022581374.1	DUF1482 family protein	-
CLC88_RS07010	1335623..1335811	-	189	WP_000450222.1	cell division inhibitor	-
CLC88_RS07020	1336596..1336964	-	369	WP_028985920.1	hypothetical protein	-
CLC88_RS07025	1336976..1337128	-	153	WP_000380321.1	DUF1391 family protein	-
CLC88_RS07030	1337400..1337687	-	288	WP_001444087.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLC88_RS07035	1337691..1337879	-	189	WP_001090455.1	hypothetical protein	Antitoxin
CLC88_RS07040	1337909..1338307	-	399	WP_000209117.1	helix-turn-helix domain-containing protein	-
CLC88_RS07045	1338424..1338702	+	279	WP_155704953.1	helix-turn-helix domain-containing protein	-
CLC88_RS07050	1338686..1339207	+	522	WP_000705386.1	hypothetical protein	-
CLC88_RS07055	1339188..1340153	+	966	WP_000054520.1	hypothetical protein	-
CLC88_RS07060	1340160..1340900	+	741	WP_000790459.1	ATP-binding protein	-
CLC88_RS07065	1340930..1341691	+	762	WP_064506729.1	DUF1627 domain-containing protein	-
CLC88_RS07070	1341724..1342005	+	282	WP_072094476.1	DNA-binding protein	-
CLC88_RS07075	1342002..1342307	+	306	WP_053294093.1	DUF4406 domain-containing protein	-
CLC88_RS07080	1342455..1342637	+	183	WP_001224672.1	hypothetical protein	-
CLC88_RS25645	1342630..1342806	+	177	WP_032295776.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2A / stx2B / stx2A / stx2B	1327257..1443187	115930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11014.71 Da
• Isoelectric Point: 6.2118

>T80551 WP_001444087.1 NZ_CP023165:c1337687-1337400 [Escherichia coli O22:H8]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVATLSIE
IVSVVHARRQFPKQN

Nucleotide

• Length: 288 bp

>T80551 NZ_CP023165:c1337687-1337400 [Escherichia coli O22:H8]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCAGAACGCTTATGGCAGCGATTAAGGGGGTGTGTGCTGCCGTTATCCGAACACCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCTCACCCTAACTATATAATTCTTTACCGTGTAGCCACTTTAAGCATTGAG
ATCGTGAGTGTAGTACACGCCAGACGCCAATTCCCCAAGCAGAACTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7249.25 Da
• Isoelectric Point: 8.6832

>AT80551 WP_001090455.1 NZ_CP023165:c1337879-1337691 [Escherichia coli O22:H8]
MNTALSPMVSEFETIEQENSYNEWLRAKVAASLANPRPAIPHDEVERRMAERFAKMRKERSK

Nucleotide

• Length: 189 bp

>AT80551 NZ_CP023165:c1337879-1337691 [Escherichia coli O22:H8]
ATGAATACGGCCCTTTCACCAATGGTTTCTGAGTTCGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAAATCCGCGCCCAGCAATTCCCCATGACGAGGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGTAAGTAG

Structures

Toxin

• AlphaFold DB: A0A234Y3J1

Antitoxin

• AlphaFold DB: A0A234Y267