Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 549655..550273 Replicon chromosome
Accession NZ_CP023165
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CLC88_RS03205 Protein ID WP_001291435.1
Coordinates 549655..549873 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CLC88_RS03210 Protein ID WP_000344800.1
Coordinates 549899..550273 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS03170 544944..545516 + 573 WP_155704909.1 YbaY family lipoprotein -
CLC88_RS03175 545547..545858 - 312 WP_000409911.1 MGMT family protein -
CLC88_RS03185 546237..546590 + 354 WP_000878141.1 DUF1428 family protein -
CLC88_RS03190 546632..548182 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CLC88_RS03195 548346..548816 - 471 WP_000136192.1 YlaC family protein -
CLC88_RS03200 548932..549483 - 552 WP_000102564.1 maltose O-acetyltransferase -
CLC88_RS03205 549655..549873 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CLC88_RS03210 549899..550273 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CLC88_RS03215 550819..553968 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CLC88_RS03220 553991..555184 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T80550 WP_001291435.1 NZ_CP023165:c549873-549655 [Escherichia coli O22:H8]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T80550 NZ_CP023165:c549873-549655 [Escherichia coli O22:H8]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT80550 WP_000344800.1 NZ_CP023165:c550273-549899 [Escherichia coli O22:H8]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT80550 NZ_CP023165:c550273-549899 [Escherichia coli O22:H8]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References