Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107609..108134 Replicon plasmid pRM10809-3
Accession NZ_CP023164
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CLC88_RS00575 Protein ID WP_001159871.1
Coordinates 107609..107914 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag CLC88_RS00580 Protein ID WP_000813639.1
Coordinates 107916..108134 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS00540 102876..104146 + 1271 Protein_106 translesion error-prone DNA polymerase V subunit UmuC -
CLC88_RS00550 104151..104543 - 393 WP_000340836.1 plasmid partitioning/stability family protein -
CLC88_RS00555 104548..105519 - 972 WP_001103695.1 hypothetical protein -
CLC88_RS00560 105748..106392 + 645 WP_000633912.1 AAA family ATPase -
CLC88_RS00565 106386..106661 + 276 WP_000239527.1 hypothetical protein -
CLC88_RS00570 106799..107608 - 810 WP_000016958.1 site-specific integrase -
CLC88_RS00575 107609..107914 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CLC88_RS00580 107916..108134 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CLC88_RS00585 108730..108990 + 261 WP_000343084.1 hypothetical protein -
CLC88_RS00590 108987..109577 + 591 WP_000194564.1 hypothetical protein -
CLC88_RS00595 109595..109941 - 347 Protein_116 hypothetical protein -
CLC88_RS00600 110125..110403 + 279 WP_001365566.1 hypothetical protein -
CLC88_RS00605 110410..110745 - 336 Protein_118 colicin -
CLC88_RS00610 110840..111570 - 731 Protein_119 complement resistance protein TraT -
CLC88_RS25600 111847..111957 - 111 Protein_120 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / espP / hlyD / hlyB / hlyA / hlyC 1..122641 122641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T80547 WP_001159871.1 NZ_CP023164:c107914-107609 [Escherichia coli O22:H8]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T80547 NZ_CP023164:c107914-107609 [Escherichia coli O22:H8]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT80547 WP_000813639.1 NZ_CP023164:c108134-107916 [Escherichia coli O22:H8]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT80547 NZ_CP023164:c108134-107916 [Escherichia coli O22:H8]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References