Detailed information of TA system TA080534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 8996..9536
• Replicon: plasmid unnamed2
• Accession: NZ_CP023157
• Organism: Xanthomonas citri pv. malvacearum strain AR81009

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CIW71_RS24415
• Protein ID: WP_007968778.1
• Coordinates: 9210..9536 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CIW71_RS24410
• Protein ID: WP_039582726.1
• Coordinates: 8996..9223 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW71_RS24380	4792..5814	+	1023	WP_007968768.1	P-type DNA transfer ATPase VirB11	-
CIW71_RS24385	5825..6712	+	888	WP_039577931.1	lytic transglycosylase domain-containing protein	-
CIW71_RS24390	6758..7345	+	588	WP_017161637.1	hypothetical protein	-
CIW71_RS24395	7390..7965	+	576	WP_039582731.1	phospholipase D family protein	-
CIW71_RS24400	7976..8389	+	414	WP_039582729.1	hypothetical protein	-
CIW71_RS24405	8443..8859	+	417	WP_017159868.1	hypothetical protein	-
CIW71_RS24410	8996..9223	+	228	WP_039582726.1	antitoxin MazE family protein	Antitoxin
CIW71_RS24415	9210..9536	+	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CIW71_RS24420	9582..10226	-	645	WP_039580900.1	Abi family protein	-
CIW71_RS24435	10773..11168	-	396	WP_046830812.1	hypothetical protein	-
CIW71_RS24440	11527..11973	+	447	WP_017160486.1	hypothetical protein	-
CIW71_RS24445	11982..12677	+	696	WP_017154898.1	hypothetical protein	-
CIW71_RS24450	12725..13078	+	354	WP_017161436.1	hypothetical protein	-
CIW71_RS24455	13216..13497	-	282	WP_017158383.1	type II toxin-antitoxin system YafQ family toxin	-
CIW71_RS24460	13484..13753	-	270	WP_017158384.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..22827	22827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T80534 WP_007968778.1 NZ_CP023157:9210-9536 [Xanthomonas citri pv. malvacearum]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T80534 NZ_CP023157:9210-9536 [Xanthomonas citri pv. malvacearum]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8804.90 Da
• Isoelectric Point: 5.0860

>AT80534 WP_039582726.1 NZ_CP023157:8996-9223 [Xanthomonas citri pv. malvacearum]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTDDAR

Nucleotide

• Length: 228 bp

>AT80534 NZ_CP023157:8996-9223 [Xanthomonas citri pv. malvacearum]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGATGATGCGAGGTGA