Detailed information of TA system TA080528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 217839..218380
• Replicon: chromosome
• Accession: NZ_CP023155
• Organism: Xanthomonas citri pv. malvacearum strain AR81009

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CIW71_RS00975
• Protein ID: WP_003491151.1
• Coordinates: 218054..218380 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: CIW71_RS00970
• Protein ID: WP_003491150.1
• Coordinates: 217839..218057 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW71_RS00945	213555..214775	+	1221	WP_078566253.1	TrbI/VirB10 family protein	-
CIW71_RS00950	214786..215808	+	1023	WP_078566254.1	P-type DNA transfer ATPase VirB11	-
CIW71_RS00955	215819..216688	+	870	WP_078566255.1	transglycosylase SLT domain-containing protein	-
CIW71_RS00960	216814..217233	+	420	WP_078566256.1	hypothetical protein	-
CIW71_RS00965	217310..217696	+	387	WP_003491149.1	hypothetical protein	-
CIW71_RS00970	217839..218057	+	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
CIW71_RS00975	218054..218380	+	327	WP_003491151.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CIW71_RS00980	218449..221424	-	2976	WP_089504402.1	conjugative relaxase	-
CIW71_RS00985	221438..223012	-	1575	WP_095702034.1	type IV secretion system DNA-binding domain-containing protein	-
CIW71_RS25090	223016..223189	-	174	WP_164722239.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	206177..228925	22748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11690.73 Da
• Isoelectric Point: 9.2550

>T80528 WP_003491151.1 NZ_CP023155:218054-218380 [Xanthomonas citri pv. malvacearum]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITIHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T80528 NZ_CP023155:218054-218380 [Xanthomonas citri pv. malvacearum]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGCATTACCA
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT80528 WP_003491150.1 NZ_CP023155:217839-218057 [Xanthomonas citri pv. malvacearum]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT80528 NZ_CP023155:217839-218057 [Xanthomonas citri pv. malvacearum]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7