Detailed information of TA system TA080526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 576881..577497
• Replicon: chromosome
• Accession: NZ_CP023154
• Organism: Pyrococcus furiosus DSM 3638

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I6U642
• Locus tag: PFDSM3638_RS02880
• Protein ID: WP_011011692.1
• Coordinates: 577096..577497 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I6UNL1
• Locus tag: PFDSM3638_RS02875
• Protein ID: WP_011011691.1
• Coordinates: 576881..577108 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFDSM3638_RS10685	572682..573098	-	417	WP_014835169.1	DUF1156 domain-containing protein	-
PFDSM3638_RS02860 (PFDSM3638_02855)	573158..573583	+	426	WP_011011687.1	DUF365 domain-containing protein	-
PFDSM3638_RS10690	573564..573980	-	417	WP_011011688.1	hypothetical protein	-
PFDSM3638_RS10695	574007..574723	-	717	WP_223209002.1	hypothetical protein	-
PFDSM3638_RS02870 (PFDSM3638_02865)	574837..576804	+	1968	WP_014835170.1	IGHMBP2 family helicase	-
PFDSM3638_RS02875 (PFDSM3638_02870)	576881..577108	+	228	WP_011011691.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PFDSM3638_RS02880 (PFDSM3638_02875)	577096..577497	+	402	WP_011011692.1	PIN domain-containing protein	Toxin
PFDSM3638_RS10700	577644..577739	-	96	WP_237718490.1	hypothetical protein	-
PFDSM3638_RS02890 (PFDSM3638_02885)	577956..578294	-	339	WP_011011693.1	PIN domain-containing protein	-
PFDSM3638_RS02895 (PFDSM3638_02890)	578281..578473	-	193	Protein_566	DUF104 domain-containing protein	-
PFDSM3638_RS02900 (PFDSM3638_02895)	578584..579435	+	852	WP_011011695.1	ABC transporter ATP-binding protein	-
PFDSM3638_RS02905 (PFDSM3638_02900)	579432..580133	+	702	WP_011011696.1	hypothetical protein	-
PFDSM3638_RS02910 (PFDSM3638_02905)	580130..580822	+	693	WP_011011697.1	permease	-
PFDSM3638_RS02915 (PFDSM3638_02910)	581083..582084	+	1002	WP_011011698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (5-48)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15632.01 Da
• Isoelectric Point: 6.4890

>T80526 WP_011011692.1 NZ_CP023154:577096-577497 [Pyrococcus furiosus DSM 3638]
MHAVIDTNVLIYDTFSDSEFHKESRSLLNSLDRWYIPSIVLQEYVWFFRSQGFSSREAKIMLSEYISDPRFRGLVEDHNV
ILRAIDILERENLSLSRFNDMIILVHAIEKGTLATFDQKLRKLARKLSVEILP

Nucleotide

• Length: 402 bp

>T80526 NZ_CP023154:577096-577497 [Pyrococcus furiosus DSM 3638]
ATGCATGCAGTGATAGATACAAATGTTCTAATTTATGATACATTTAGCGATTCTGAGTTTCACAAAGAGTCTAGATCTCT
TTTGAATTCTCTTGATAGATGGTACATTCCTTCCATAGTACTTCAGGAGTATGTGTGGTTTTTTAGAAGTCAAGGATTTT
CAAGTAGAGAGGCCAAGATAATGCTCTCTGAATATATATCAGATCCAAGATTCAGAGGTCTTGTAGAAGACCACAATGTA
ATTCTTCGTGCCATTGACATTCTCGAAAGGGAAAACCTCTCTTTATCACGGTTTAATGATATGATAATTCTTGTTCATGC
AATAGAGAAGGGTACCCTAGCAACTTTTGATCAAAAACTCAGAAAACTTGCAAGGAAGTTGAGCGTTGAAATCTTGCCAT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8705.47 Da
• Isoelectric Point: 10.2059

>AT80526 WP_011011691.1 NZ_CP023154:576881-577108 [Pyrococcus furiosus DSM 3638]
MPITKVTRNYQITIPAEIRKALGIKEGELLEVRLENGKIIIERLKKERKTLKLGKKLTLEEIEKAIEEGMKQCMQ

Nucleotide

• Length: 228 bp

>AT80526 NZ_CP023154:576881-577108 [Pyrococcus furiosus DSM 3638]
ATGCCCATTACGAAGGTTACTAGAAACTATCAGATAACAATTCCTGCAGAGATTAGAAAGGCCCTTGGAATTAAAGAAGG
TGAACTGCTTGAAGTAAGGCTAGAAAATGGAAAAATAATTATAGAAAGACTCAAAAAAGAAAGAAAAACACTCAAGCTGG
GTAAAAAGTTAACACTCGAAGAAATTGAGAAAGCGATAGAGGAGGGAATGAAACAATGCATGCAGTGA

Structures

Toxin

• AlphaFold DB: I6U642

Antitoxin

• AlphaFold DB: I6UNL1