Detailed information of TA system TA080522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 13898..14424
• Replicon: plasmid pFLAC0070_1
• Accession: NZ_CP023152
• Organism: Mycobacterium intracellulare subsp. chimaera strain FLAC0070

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: CKJ58_RS25730
• Protein ID: WP_095663108.1
• Coordinates: 14122..14424 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: -
• Locus tag: CKJ58_RS25725
• Protein ID: WP_095663107.1
• Coordinates: 13898..14125 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKJ58_RS25695	10578..10994	-	417	WP_095663104.1	hypothetical protein	-
CKJ58_RS25700	10997..11581	-	585	WP_176476823.1	hypothetical protein	-
CKJ58_RS25705	11574..11780	-	207	WP_095663105.1	hypothetical protein	-
CKJ58_RS25710	11830..12087	-	258	WP_141244907.1	hypothetical protein	-
CKJ58_RS25715	12273..13401	+	1129	Protein_13	hypothetical protein	-
CKJ58_RS25720	13475..13726	+	252	WP_095663208.1	helix-turn-helix transcriptional regulator	-
CKJ58_RS25725	13898..14125	+	228	WP_095663107.1	ribbon-helix-helix domain-containing protein	Antitoxin
CKJ58_RS25730	14122..14424	+	303	WP_095663108.1	toxin	Toxin
CKJ58_RS25740	15308..15982	+	675	WP_095663209.1	Crp/Fnr family transcriptional regulator	-
CKJ58_RS25745	16264..16929	-	666	WP_095663110.1	RES family NAD+ phosphorylase	-
CKJ58_RS25750	16926..17513	-	588	WP_095663111.1	hypothetical protein	-
CKJ58_RS25755	18180..18539	-	360	WP_023870411.1	metalloregulator ArsR/SmtB family transcription factor	-
CKJ58_RS25760	18642..19121	+	480	WP_023870410.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	esxN	1..247522	247522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10373.13 Da
• Isoelectric Point: 4.3271

>T80522 WP_095663108.1 NZ_CP023152:14122-14424 [Mycobacterium intracellulare subsp. chimaera]
VIAPGDITPRRDTDQELYVVILSNAIHLAAATGRVITCPFIPGEIPSGTMAMIVTVQRPKGVVLPELIQWLPVAALDEPM
GNIGGAALADTTATVTALVS

Nucleotide

• Length: 303 bp

>T80522 NZ_CP023152:14122-14424 [Mycobacterium intracellulare subsp. chimaera]
GTGATCGCACCCGGAGACATCACGCCACGCCGCGACACCGACCAGGAACTCTACGTGGTCATACTGTCTAACGCGATTCA
CCTCGCAGCCGCTACTGGGCGAGTGATCACCTGCCCGTTTATTCCCGGTGAAATCCCTAGCGGCACAATGGCTATGATCG
TCACAGTGCAGCGACCCAAAGGGGTTGTCTTGCCCGAACTGATTCAGTGGCTTCCTGTCGCGGCCCTCGACGAACCCATG
GGCAATATCGGCGGCGCAGCGCTCGCCGATACGACAGCGACCGTCACTGCCCTCGTTTCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8198.23 Da
• Isoelectric Point: 5.6306

>AT80522 WP_095663107.1 NZ_CP023152:13898-14125 [Mycobacterium intracellulare subsp. chimaera]
MAKDKVSVTIDQDVLAAADVDAQGAGLNRSEMIERALRNEHLRISLENYTTHTVPALDIDAYAKKVYQANRAAGL

Nucleotide

• Length: 228 bp

>AT80522 NZ_CP023152:13898-14125 [Mycobacterium intracellulare subsp. chimaera]
ATGGCGAAAGATAAGGTCTCGGTGACGATCGATCAGGATGTACTGGCGGCGGCAGATGTGGACGCCCAAGGGGCCGGACT
GAATCGTTCAGAGATGATCGAACGGGCCCTGCGCAACGAGCATCTCCGAATCTCGCTCGAGAACTACACCACACACACTG
TTCCAGCGCTCGATATCGACGCCTACGCAAAGAAGGTTTATCAGGCGAATCGGGCCGCTGGCCTGTGA