Detailed information of TA system TA080521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 3877411..3877937
• Replicon: chromosome
• Accession: NZ_CP023151
• Organism: Mycobacterium intracellulare subsp. chimaera strain FLAC0070

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: S4ZDZ6
• Locus tag: CKJ58_RS18190
• Protein ID: WP_008259048.1
• Coordinates: 3877635..3877937 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: S4ZFD0
• Locus tag: CKJ58_RS18185
• Protein ID: WP_008259047.1
• Coordinates: 3877411..3877638 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKJ58_RS18160	3872488..3873408	-	921	WP_008259041.1	MinD/ParA family protein	-
CKJ58_RS18165	3873653..3874684	-	1032	WP_008259042.1	6-phosphofructokinase	-
CKJ58_RS18170	3874728..3876221	-	1494	WP_008259043.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
CKJ58_RS18175	3876218..3876517	-	300	WP_008259044.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
CKJ58_RS18180	3876617..3877273	+	657	WP_085976512.1	amino acid-binding protein	-
CKJ58_RS18185	3877411..3877638	+	228	WP_008259047.1	ribbon-helix-helix protein, CopG family	Antitoxin
CKJ58_RS18190	3877635..3877937	+	303	WP_008259048.1	hypothetical protein	Toxin
CKJ58_RS18195	3878053..3878361	-	309	WP_008259049.1	hypothetical protein	-
CKJ58_RS18200	3878420..3881341	-	2922	WP_014712101.1	MMPL family transporter	-
CKJ58_RS18205	3881338..3881784	-	447	WP_193375010.1	transport acessory protein MmpS	-
CKJ58_RS18210	3881989..3882507	+	519	WP_014712102.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10473.31 Da
• Isoelectric Point: 4.5636

>T80521 WP_008259048.1 NZ_CP023151:3877635-3877937 [Mycobacterium intracellulare subsp. chimaera]
MIAPGDIAPRRDTRRDLYVAVLSNAIHLAANTGRVIVCPFIPGKIPDDTMAMVVPLTQPEGTLLPELVQWLPTSALGEPI
GAVSAAALSEAMSIVTALIT

Nucleotide

• Length: 303 bp

>T80521 NZ_CP023151:3877635-3877937 [Mycobacterium intracellulare subsp. chimaera]
ATGATCGCGCCGGGCGACATCGCGCCGCGGCGAGACACCCGCCGCGACCTCTACGTCGCGGTGCTCTCCAACGCGATCCA
CCTCGCGGCCAACACTGGTCGCGTCATCGTCTGCCCGTTCATCCCTGGGAAAATCCCCGACGACACGATGGCCATGGTCG
TTCCGCTCACGCAGCCCGAAGGCACTCTTCTCCCCGAACTGGTGCAGTGGCTGCCGACTTCGGCGCTCGGTGAACCGATC
GGGGCCGTGTCGGCGGCCGCGCTTAGCGAGGCGATGTCGATCGTGACCGCGCTGATCACGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.14 Da
• Isoelectric Point: 6.4608

>AT80521 WP_008259047.1 NZ_CP023151:3877411-3877638 [Mycobacterium intracellulare subsp. chimaera]
MAKEKISVTVDAAVLADADSDAKAAGLNRSEMIERALRNEHLRRALHTYTTRTAPALNIDSYAGQVYEANRAADL

Nucleotide

• Length: 228 bp

>AT80521 NZ_CP023151:3877411-3877638 [Mycobacterium intracellulare subsp. chimaera]
ATGGCCAAAGAGAAGATTTCTGTCACCGTCGACGCCGCCGTGCTCGCCGACGCCGACTCCGACGCCAAAGCGGCCGGACT
GAATCGCTCCGAGATGATCGAGCGGGCACTGCGCAATGAGCATCTACGTCGCGCGCTTCATACCTATACGACGCGGACCG
CACCAGCGCTCAACATCGACTCCTACGCCGGGCAAGTGTATGAAGCCAACCGGGCCGCGGACCTATGA

Structures

Toxin

• AlphaFold DB: S4ZDZ6

Antitoxin

• AlphaFold DB: S4ZFD0