Detailed information of TA system TA080516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1151036..1151600
• Replicon: chromosome
• Accession: NZ_CP023149
• Organism: Mycobacterium intracellulare strain FLAC0181

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IVI4
• Locus tag: CKJ67_RS05830
• Protein ID: WP_009953437.1
• Coordinates: 1151229..1151600 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CKJ67_RS05825
• Protein ID: WP_026071432.1
• Coordinates: 1151036..1151242 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKJ67_RS05805	1146657..1147655	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CKJ67_RS05810	1147701..1148840	-	1140	WP_179300624.1	hypothetical protein	-
CKJ67_RS05815	1148982..1150055	+	1074	WP_009953440.1	redox-regulated ATPase YchF	-
CKJ67_RS05820	1150135..1150797	-	663	WP_014379386.1	methyltransferase domain-containing protein	-
CKJ67_RS05825	1151036..1151242	+	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CKJ67_RS05830	1151229..1151600	+	372	WP_009953437.1	type II toxin-antitoxin system VapC family toxin	Toxin
CKJ67_RS05835	1151658..1152209	+	552	WP_009953436.1	VOC family protein	-
CKJ67_RS05840	1152288..1152686	+	399	WP_009953435.1	VOC family protein	-
CKJ67_RS05845	1152785..1153168	+	384	WP_026071433.1	hypothetical protein	-
CKJ67_RS26905	1153254..1153613	+	360	WP_179293417.1	hypothetical protein	-
CKJ67_RS05855	1153795..1154370	+	576	WP_085977620.1	C40 family peptidase	-
CKJ67_RS05860	1154733..1155056	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-
CKJ67_RS05865	1155105..1155788	-	684	WP_014384004.1	hypothetical protein	-
CKJ67_RS05870	1155935..1156144	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T80516 WP_009953437.1 NZ_CP023149:1151229-1151600 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEQRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T80516 NZ_CP023149:1151229-1151600 [Mycobacterium intracellulare]
GTGATCCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCAGCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCATCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGTGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAAAGAAGTGGGTGTTGCGATCGTCGATGAGTCGTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT80516 WP_026071432.1 NZ_CP023149:1151036-1151242 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT80516 NZ_CP023149:1151036-1151242 [Mycobacterium intracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGACGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATCCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7R7MQX9