Detailed information of TA system TA080512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2591040..2592032
• Replicon: chromosome
• Accession: NZ_CP023146
• Organism: Mycobacterium intracellulare strain FLAC0133

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: CKJ61_RS12320
• Protein ID: WP_038536181.1
• Coordinates: 2591454..2592032 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: X8A634
• Locus tag: CKJ61_RS12315
• Protein ID: WP_038536179.1
• Coordinates: 2591040..2591429 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKJ61_RS12290	2586491..2588494	+	2004	WP_095562692.1	phosphotransferase	-
CKJ61_RS12295	2588491..2588991	+	501	WP_038536171.1	gluconokinase	-
CKJ61_RS12300	2589062..2589673	-	612	WP_095562693.1	TetR/AcrR family transcriptional regulator	-
CKJ61_RS12305	2589865..2590668	+	804	WP_009956382.1	SAM-dependent methyltransferase	-
CKJ61_RS12310	2590708..2590983	+	276	WP_038536176.1	hypothetical protein	-
CKJ61_RS12315	2591040..2591429	+	390	WP_038536179.1	hypothetical protein	Antitoxin
CKJ61_RS12320	2591454..2592032	+	579	WP_038536181.1	zeta toxin family protein	Toxin
CKJ61_RS12325	2592052..2593119	-	1068	WP_038536184.1	DoxX family membrane protein	-
CKJ61_RS12330	2593215..2593775	+	561	WP_042911761.1	pyrazinamidase PncA	-
CKJ61_RS12335	2593775..2594575	+	801	WP_009956375.1	nuclear transport factor 2 family protein	-
CKJ61_RS12340	2594587..2594937	-	351	WP_020188920.1	DoxX family protein	-
CKJ61_RS12345	2595103..2596074	+	972	WP_014382494.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-137)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21103.22 Da
• Isoelectric Point: 11.0233

>T80512 WP_038536181.1 NZ_CP023146:2591454-2592032 [Mycobacterium intracellulare]
MQRLDLVVGPNGAGKSTFIPFTLAPLLPGSPVVNADEIARQRWPGESAGRAYDAAQIAAATRAKLIDLGRSFIAETVFSH
PSKLDLIRTAHRAGFTVVLHAMLIPEDLAVERVRHRVRAGGHDVPETKVRERHRRLWTPVTDAIALSDVATVYDNSRLRG
PRIVARLSGGMTIGSPDWPAWAPEILRSRWPD

Nucleotide

• Length: 579 bp

>T80512 NZ_CP023146:2591454-2592032 [Mycobacterium intracellulare]
ATGCAGCGACTTGACCTGGTGGTCGGGCCGAACGGTGCCGGCAAGTCGACCTTCATCCCGTTCACCCTGGCGCCGCTGTT
GCCCGGCAGCCCGGTCGTCAACGCCGACGAAATCGCAAGGCAGCGCTGGCCGGGGGAGTCGGCAGGGCGCGCCTACGACG
CCGCGCAAATCGCCGCCGCCACGCGCGCGAAGTTGATCGACCTGGGGCGGTCGTTCATCGCGGAGACCGTGTTCTCCCAC
CCGTCGAAGCTGGACCTCATCCGCACCGCCCACCGAGCGGGCTTCACCGTCGTCCTGCACGCGATGCTCATCCCGGAAGA
CCTTGCCGTCGAACGTGTCCGGCACCGCGTGCGGGCCGGCGGCCACGACGTACCGGAAACCAAGGTTCGCGAGCGCCATC
GTCGCTTGTGGACACCGGTCACCGACGCCATCGCGCTTTCCGACGTCGCCACCGTCTACGACAACAGCCGGCTGCGCGGG
CCGCGCATTGTCGCCCGCCTCAGCGGCGGCATGACGATCGGTTCACCGGACTGGCCGGCGTGGGCTCCGGAGATCTTGCG
GTCGCGCTGGCCGGACTAG

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13507.88 Da
• Isoelectric Point: 5.5186

>AT80512 WP_038536179.1 NZ_CP023146:2591040-2591429 [Mycobacterium intracellulare]
MADAPDRVTRVASDLMDSAASEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGHLATGELTVEEGVVFNAEI
SAAIDESLVHADYGATLAGHGVTTVALNEDGDIVEHRPDGAAVVLARGR

Nucleotide

• Length: 390 bp

>AT80512 NZ_CP023146:2591040-2591429 [Mycobacterium intracellulare]
ATGGCCGACGCTCCCGATCGTGTGACCCGCGTGGCGTCCGACCTGATGGACAGCGCGGCCTCCGAAGGCGCCCGCCAGAG
CCGTTCGGCCAAGCAGCAACTCGACCACTGGGCGCGTGTCGGGCGCGCGGTGTCCAGCCAGCACACCGCCTCGCGTCGGC
GGGTCGAGGCCGCGCTGGCCGGCCACCTGGCGACCGGCGAACTCACCGTCGAGGAAGGCGTGGTGTTCAACGCCGAGATC
TCCGCCGCCATCGACGAAAGCCTGGTGCACGCCGACTACGGGGCGACGCTGGCCGGGCATGGCGTCACCACGGTCGCCCT
CAACGAGGACGGCGACATCGTCGAGCACCGGCCCGATGGCGCCGCCGTGGTGCTGGCGCGCGGGCGCTGA

Structures

Antitoxin

• AlphaFold DB: X8A634