Detailed information of TA system TA080511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1121593..1122157
• Replicon: chromosome
• Accession: NZ_CP023146
• Organism: Mycobacterium intracellulare strain FLAC0133

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD68
• Locus tag: CKJ61_RS05665
• Protein ID: WP_014941408.1
• Coordinates: 1121786..1122157 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CKJ61_RS05660
• Protein ID: WP_038538466.1
• Coordinates: 1121593..1121799 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKJ61_RS05640	1117208..1118206	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CKJ61_RS05645	1118252..1119397	-	1146	WP_198347161.1	hypothetical protein	-
CKJ61_RS05650	1119539..1120612	+	1074	WP_009953440.1	redox-regulated ATPase YchF	-
CKJ61_RS05655	1120692..1121354	-	663	WP_014379386.1	methyltransferase domain-containing protein	-
CKJ61_RS05660	1121593..1121799	+	207	WP_038538466.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CKJ61_RS05665	1121786..1122157	+	372	WP_014941408.1	type II toxin-antitoxin system VapC family toxin	Toxin
CKJ61_RS05670	1122215..1122766	+	552	WP_038535279.1	VOC family protein	-
CKJ61_RS05675	1122845..1123243	+	399	WP_009953435.1	VOC family protein	-
CKJ61_RS05680	1123390..1123725	+	336	WP_038535281.1	hypothetical protein	-
CKJ61_RS26010	1123811..1124170	+	360	WP_179960061.1	hypothetical protein	-
CKJ61_RS05690	1124352..1124927	+	576	WP_085977620.1	C40 family peptidase	-
CKJ61_RS05695	1125290..1125613	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-
CKJ61_RS05700	1125662..1126345	-	684	WP_014384004.1	hypothetical protein	-
CKJ61_RS05705	1126492..1126701	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T80511 WP_014941408.1 NZ_CP023146:1121786-1122157 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEHRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSQLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T80511 NZ_CP023146:1121786-1122157 [Mycobacterium intracellulare]
GTGATTCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCATCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCAGCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGTGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAAAGAAGTGGGTGTTGCGATCGTCGATGAGTCGTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7240.27 Da
• Isoelectric Point: 11.9169

>AT80511 WP_038538466.1 NZ_CP023146:1121593-1121799 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDSR

Nucleotide

• Length: 207 bp

>AT80511 NZ_CP023146:1121593-1121799 [Mycobacterium intracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGACGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: J9WD68