Detailed information of TA system TA080510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 884593..885246
• Replicon: chromosome
• Accession: NZ_CP023146
• Organism: Mycobacterium intracellulare strain FLAC0133

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CKJ61_RS04550
• Protein ID: WP_008253911.1
• Coordinates: 884785..885246 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: CKJ61_RS04545
• Protein ID: WP_009957321.1
• Coordinates: 884593..884781 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKJ61_RS04535	880566..882143	+	1578	WP_014381859.1	serine hydrolase	-
CKJ61_RS04540	882140..884527	+	2388	WP_095562464.1	cation-translocating P-type ATPase	-
CKJ61_RS04545	884593..884781	+	189	WP_009957321.1	antitoxin	Antitoxin
CKJ61_RS04550	884785..885246	+	462	WP_008253911.1	SRPBCC family protein	Toxin
CKJ61_RS04555	885333..886103	+	771	WP_095562465.1	VOC family protein	-
CKJ61_RS04560	886177..886611	+	435	WP_008253914.1	hypothetical protein	-
CKJ61_RS04565	886616..887104	-	489	WP_036458243.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
CKJ61_RS04570	887104..888630	-	1527	WP_038535114.1	carotenoid oxygenase family protein	-
CKJ61_RS04575	888632..889852	-	1221	WP_036428523.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T80510 WP_008253911.1 NZ_CP023146:884785-885246 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T80510 NZ_CP023146:884785-885246 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCTGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT80510 WP_009957321.1 NZ_CP023146:884593-884781 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT80510 NZ_CP023146:884593-884781 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAGTACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: X8A187