Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 73633..74158 Replicon plasmid pEGY3
Accession NZ_CP023145
Organism Escherichia coli strain CFSAN061770

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CA696_RS30055 Protein ID WP_001159868.1
Coordinates 73853..74158 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CA696_RS30050 Protein ID WP_000813634.1
Coordinates 73633..73851 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA696_RS30020 69026..69442 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
CA696_RS30025 69439..69669 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CA696_RS30030 69934..70434 + 501 WP_000528931.1 hypothetical protein -
CA696_RS30035 70447..71220 + 774 WP_000905949.1 hypothetical protein -
CA696_RS30040 71387..72520 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
CA696_RS30045 72554..73066 - 513 WP_000151784.1 hypothetical protein -
CA696_RS30050 73633..73851 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CA696_RS30055 73853..74158 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CA696_RS30060 74159..74965 + 807 WP_000016982.1 site-specific integrase -
CA696_RS30065 75739..76494 + 756 WP_000852146.1 replication initiation protein RepE -
CA696_RS30070 77082..78248 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / aadA5 / sitABCD iucA / iucB / iucC / iucD / iutA 1..87173 87173


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T80509 WP_001159868.1 NZ_CP023145:73853-74158 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T80509 NZ_CP023145:73853-74158 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT80509 WP_000813634.1 NZ_CP023145:73633-73851 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT80509 NZ_CP023145:73633-73851 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References