Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69026..69669 Replicon plasmid pEGY3
Accession NZ_CP023145
Organism Escherichia coli strain CFSAN061770

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag CA696_RS30020 Protein ID WP_001034046.1
Coordinates 69026..69442 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag CA696_RS30025 Protein ID WP_001261278.1
Coordinates 69439..69669 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA696_RS30005 64163..64579 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CA696_RS30010 64576..64806 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CA696_RS30015 65187..68981 + 3795 WP_001144732.1 hypothetical protein -
CA696_RS30020 69026..69442 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
CA696_RS30025 69439..69669 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CA696_RS30030 69934..70434 + 501 WP_000528931.1 hypothetical protein -
CA696_RS30035 70447..71220 + 774 WP_000905949.1 hypothetical protein -
CA696_RS30040 71387..72520 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
CA696_RS30045 72554..73066 - 513 WP_000151784.1 hypothetical protein -
CA696_RS30050 73633..73851 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
CA696_RS30055 73853..74158 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / aadA5 / sitABCD iucA / iucB / iucC / iucD / iutA 1..87173 87173


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T80508 WP_001034046.1 NZ_CP023145:c69442-69026 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T80508 NZ_CP023145:c69442-69026 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT80508 WP_001261278.1 NZ_CP023145:c69669-69439 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT80508 NZ_CP023145:c69669-69439 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References