Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 36680..37239 Replicon plasmid pEGY3
Accession NZ_CP023145
Organism Escherichia coli strain CFSAN061770

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CA696_RS29820 Protein ID WP_000421272.1
Coordinates 36964..37239 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CA696_RS29815 Protein ID WP_001387467.1
Coordinates 36680..36964 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA696_RS29785 31971..33134 - 1164 Protein_39 IS4-like element ISVsa5 family transposase -
CA696_RS29790 33149..33846 - 698 Protein_40 IS1 family transposase -
CA696_RS29800 33896..34279 + 384 WP_032338434.1 Rid family detoxifying hydrolase -
CA696_RS29805 34312..35277 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CA696_RS29810 35323..36075 - 753 WP_000562172.1 IclR family transcriptional regulator -
CA696_RS29815 36680..36964 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CA696_RS29820 36964..37239 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CA696_RS29825 37345..37638 + 294 WP_001105060.1 hypothetical protein -
CA696_RS29830 37673..37801 - 129 Protein_47 IS481 family transposase -
CA696_RS29835 37834..39003 - 1170 WP_001072355.1 hypothetical protein -
CA696_RS29845 39496..39803 + 308 Protein_49 type II toxin-antitoxin system ParD family antitoxin -
CA696_RS29855 40146..40328 + 183 WP_042344623.1 hypothetical protein -
CA696_RS29860 40449..41189 + 741 WP_001066953.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / aadA5 / sitABCD iucA / iucB / iucC / iucD / iutA 1..87173 87173


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T80506 WP_000421272.1 NZ_CP023145:36964-37239 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T80506 NZ_CP023145:36964-37239 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT80506 WP_001387467.1 NZ_CP023145:36680-36964 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT80506 NZ_CP023145:36680-36964 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References