Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1005898..1006552 Replicon chromosome
Accession NZ_CP023142
Organism Escherichia coli strain CFSAN061770

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag CA696_RS05245 Protein ID WP_000244769.1
Coordinates 1006145..1006552 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CA696_RS05240 Protein ID WP_000354046.1
Coordinates 1005898..1006164 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA696_RS05220 1001986..1003419 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
CA696_RS05225 1003464..1003775 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CA696_RS05230 1003939..1004598 + 660 WP_000250274.1 hemolysin III family protein -
CA696_RS05235 1004675..1005655 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
CA696_RS05240 1005898..1006164 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CA696_RS05245 1006145..1006552 + 408 WP_000244769.1 protein YgfX Toxin
CA696_RS05250 1006592..1007113 - 522 WP_001055874.1 flavodoxin FldB -
CA696_RS05255 1007225..1008121 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
CA696_RS05260 1008146..1008856 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CA696_RS05265 1008862..1010595 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T80480 WP_000244769.1 NZ_CP023142:1006145-1006552 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T80480 NZ_CP023142:1006145-1006552 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT80480 WP_000354046.1 NZ_CP023142:1005898-1006164 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT80480 NZ_CP023142:1005898-1006164 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References