Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 853316..854151 Replicon chromosome
Accession NZ_CP023142
Organism Escherichia coli strain CFSAN061770

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W3AJH4
Locus tag CA696_RS04450 Protein ID WP_000854721.1
Coordinates 853316..853693 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A6Y4TI76
Locus tag CA696_RS04455 Protein ID WP_072668294.1
Coordinates 853783..854151 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA696_RS04410 848465..848700 - 236 Protein_801 IS110 family transposase -
CA696_RS04420 849284..850549 - 1266 WP_095597201.1 integrase arm-type DNA-binding domain-containing protein -
CA696_RS04425 851013..851339 - 327 WP_095597202.1 type II toxin-antitoxin system RelE/ParE family toxin -
CA696_RS04430 851336..851599 - 264 WP_001143297.1 type II toxin-antitoxin system ParD family antitoxin -
CA696_RS04435 851671..852537 - 867 WP_095597203.1 DUF4942 domain-containing protein -
CA696_RS04440 852622..852819 - 198 WP_000839291.1 DUF957 domain-containing protein -
CA696_RS04445 852831..853319 - 489 WP_000761677.1 hypothetical protein -
CA696_RS04450 853316..853693 - 378 WP_000854721.1 TA system toxin CbtA family protein Toxin
CA696_RS04455 853783..854151 - 369 WP_072668294.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CA696_RS04460 854201..854848 - 648 WP_072668293.1 antitoxin of toxin-antitoxin stability system -
CA696_RS04465 854867..855088 - 222 WP_000692318.1 DUF987 domain-containing protein -
CA696_RS04470 855151..855627 - 477 WP_040089737.1 RadC family protein -
CA696_RS04475 855643..856128 - 486 WP_095597204.1 antirestriction protein -
CA696_RS04480 856219..857037 - 819 WP_095597205.1 DUF945 domain-containing protein -
CA696_RS04485 857223..858107 - 885 WP_000010380.1 50S ribosome-binding GTPase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 851013..922019 71006


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14089.12 Da        Isoelectric Point: 8.2904

>T80479 WP_000854721.1 NZ_CP023142:c853693-853316 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T80479 NZ_CP023142:c853693-853316 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13843.53 Da        Isoelectric Point: 5.8845

>AT80479 WP_072668294.1 NZ_CP023142:c854151-853783 [Escherichia coli]
VSDSRHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT80479 NZ_CP023142:c854151-853783 [Escherichia coli]
GTGTCAGACTCGCGCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3AJH4


Antitoxin

Source ID Structure
AlphaFold DB A0A6Y4TI76

References