Detailed information of TA system TA080459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1298123..1299040
• Replicon: chromosome
• Accession: NZ_CP023075
• Organism: Bacillus velezensis strain K26

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: CK238_RS06870
• Protein ID: WP_007407256.1
• Coordinates: 1298294..1299040 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: CK238_RS06865
• Protein ID: WP_003154807.1
• Coordinates: 1298123..1298293 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CK238_RS06820	1293329..1293841	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
CK238_RS06825	1293954..1294613	+	660	WP_017417606.1	terminase	-
CK238_RS06830	1294511..1294978	+	468	WP_076984018.1	hypothetical protein	-
CK238_RS06835	1294991..1295362	+	372	WP_038457736.1	YomQ/XkdW protein, phage-like element PBSX	-
CK238_RS06840	1295367..1295564	+	198	WP_007610833.1	XkdX family protein	-
CK238_RS06845	1295621..1296382	+	762	WP_063636818.1	hypothetical protein	-
CK238_RS06850	1296434..1296697	+	264	WP_021493821.1	hemolysin XhlA family protein	-
CK238_RS06855	1296711..1296974	+	264	WP_003154813.1	phage holin	-
CK238_RS06860	1296988..1297866	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
CK238_RS06865	1298123..1298293	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CK238_RS06870	1298294..1299040	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CK238_RS06875	1299144..1300142	-	999	WP_017417610.1	inorganic phosphate transporter	-
CK238_RS06880	1300155..1300772	-	618	WP_003154804.1	DUF47 domain-containing protein	-
CK238_RS06885	1301059..1302375	-	1317	WP_063636817.1	amino acid permease	-
CK238_RS06890	1302697..1303647	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T80459 WP_007407256.1 NZ_CP023075:c1299040-1298294 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T80459 NZ_CP023075:c1299040-1298294 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT80459 WP_003154807.1 NZ_CP023075:c1298293-1298123 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT80459 NZ_CP023075:c1298293-1298123 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14