Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 484320..484957 Replicon chromosome
Accession NZ_CP023075
Organism Bacillus velezensis strain K26

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CK238_RS02495 Protein ID WP_003156187.1
Coordinates 484607..484957 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CK238_RS02490 Protein ID WP_003156188.1
Coordinates 484320..484601 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CK238_RS02470 480685..481284 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CK238_RS02475 481377..481742 + 366 WP_014304402.1 holo-ACP synthase -
CK238_RS02480 481907..482914 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
CK238_RS02485 483031..484200 + 1170 WP_063636967.1 alanine racemase -
CK238_RS02490 484320..484601 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CK238_RS02495 484607..484957 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CK238_RS02500 485075..485896 + 822 WP_025649964.1 STAS domain-containing protein -
CK238_RS02505 485901..486266 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
CK238_RS02510 486269..486670 + 402 WP_003156178.1 anti-sigma regulatory factor -
CK238_RS02515 486682..487689 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
CK238_RS02520 487753..488082 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CK238_RS02525 488079..488561 + 483 WP_007609591.1 anti-sigma B factor RsbW -
CK238_RS02530 488527..489315 + 789 WP_063636966.1 RNA polymerase sigma factor SigB -
CK238_RS02535 489315..489917 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T80458 WP_003156187.1 NZ_CP023075:484607-484957 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T80458 NZ_CP023075:484607-484957 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT80458 WP_003156188.1 NZ_CP023075:484320-484601 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT80458 NZ_CP023075:484320-484601 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References