Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 536678..537354 Replicon plasmid pSF83666a
Accession NZ_CP023072
Organism Sinorhizobium fredii CCBAU 83666

Toxin (Protein)


Gene name vapC Uniprot ID A0A249Q7B5
Locus tag SF83666_RS33155 Protein ID WP_037427474.1
Coordinates 536678..537100 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2A6LPC6
Locus tag SF83666_RS33160 Protein ID WP_010875326.1
Coordinates 537097..537354 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF83666_RS33125 532423..532686 - 264 WP_086021547.1 Y4bD/Y4pK family protein -
SF83666_RS33130 532731..533408 + 678 Protein_491 ISNCY family transposase -
SF83666_RS33135 533776..535128 + 1353 WP_037427476.1 IS5 family transposase -
SF83666_RS33150 535637..536374 - 738 WP_095678168.1 hypothetical protein -
SF83666_RS33155 536678..537100 - 423 WP_037427474.1 type II toxin-antitoxin system VapC family toxin Toxin
SF83666_RS33160 537097..537354 - 258 WP_010875326.1 plasmid stability protein stbC Antitoxin
SF83666_RS33165 537672..537968 + 297 WP_037427472.1 DUF2604 domain-containing protein -
SF83666_RS33170 537961..538566 + 606 WP_037427479.1 hypothetical protein -
SF83666_RS33175 538563..539051 + 489 WP_037427470.1 Mov34/MPN/PAD-1 family protein -
SF83666_RS33180 539054..540613 + 1560 WP_037427469.1 E2 ligase fold family C protein -
SF83666_RS33190 541333..541590 + 258 WP_014857619.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB / gmd 1..742947 742947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15105.40 Da        Isoelectric Point: 4.5257

>T80456 WP_037427474.1 NZ_CP023072:c537100-536678 [Sinorhizobium fredii CCBAU 83666]
MIVLDTNVISELWKAEPDRAVLAWIDAQMIETLYLSAITVAELRFGLAAMPAGKRRTIFQNRLEGEVLPALAGRVLPFDL
DASRSYADLMAQAKTSGNVIGKADGYIAATAVAHGFMVATRDTSPFEAAGLDIINPWEPA

Download         Length: 423 bp

>T80456 NZ_CP023072:c537100-536678 [Sinorhizobium fredii CCBAU 83666]
ATGATCGTGCTCGACACCAACGTCATATCCGAATTGTGGAAGGCCGAGCCGGACCGCGCTGTGTTGGCCTGGATCGATGC
GCAGATGATTGAAACACTGTATTTGTCCGCGATTACGGTGGCCGAACTGCGTTTCGGCTTGGCGGCCATGCCGGCAGGCA
AGCGACGCACGATCTTTCAGAATCGCTTGGAAGGAGAGGTCTTGCCAGCCCTCGCAGGGCGCGTTCTTCCCTTCGACTTG
GACGCGTCGCGAAGTTATGCGGACCTGATGGCGCAAGCGAAAACGTCAGGCAACGTCATCGGCAAGGCGGATGGCTATAT
CGCCGCAACGGCGGTTGCGCATGGCTTTATGGTTGCTACGCGCGATACCAGTCCGTTCGAGGCAGCTGGACTAGACATCA
TCAACCCCTGGGAGCCGGCATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.65 Da        Isoelectric Point: 6.0775

>AT80456 WP_010875326.1 NZ_CP023072:c537354-537097 [Sinorhizobium fredii CCBAU 83666]
MANVTVRNLPDEVHRALRVRAAMHGRSTEAEIRDILETTVRPPERVKLGSLLASIAHEAGGLTDAEAEHFNQLRDKTPAE
PMSFE

Download         Length: 258 bp

>AT80456 NZ_CP023072:c537354-537097 [Sinorhizobium fredii CCBAU 83666]
ATGGCAAATGTGACCGTTAGGAACCTGCCCGACGAGGTGCACCGAGCGCTACGCGTACGAGCAGCAATGCATGGCCGCAG
CACCGAAGCCGAGATCCGCGACATCCTAGAAACGACTGTCCGGCCCCCGGAGCGGGTCAAACTCGGCTCTTTATTGGCGT
CTATTGCCCATGAAGCTGGAGGGCTGACGGATGCCGAAGCGGAGCATTTCAACCAGCTCCGTGACAAGACTCCTGCTGAA
CCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249Q7B5

Antitoxin

Source ID Structure
AlphaFold DB A0A2A6LPC6

References