Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 92793..94282 Replicon plasmid pSF83666a
Accession NZ_CP023072
Organism Sinorhizobium fredii CCBAU 83666

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A249Q653
Locus tag SF83666_RS30895 Protein ID WP_037436876.1
Coordinates 92793..94055 (-) Length 421 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID A0A249Q849
Locus tag SF83666_RS30900 Protein ID WP_010875278.1
Coordinates 94052..94282 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF83666_RS35280 88410..88658 + 249 WP_157750342.1 hypothetical protein -
SF83666_RS30880 89445..90255 - 811 Protein_84 IS5 family transposase -
SF83666_RS30885 90457..91023 + 567 WP_080579338.1 hypothetical protein -
SF83666_RS30890 91649..92482 + 834 WP_095689851.1 IS5 family transposase -
SF83666_RS30895 92793..94055 - 1263 WP_037436876.1 type II toxin-antitoxin system HipA family toxin Toxin
SF83666_RS30900 94052..94282 - 231 WP_010875278.1 helix-turn-helix transcriptional regulator Antitoxin
SF83666_RS30905 94820..95167 + 348 WP_162130067.1 DUF1778 domain-containing protein -
SF83666_RS30910 95516..96410 - 895 Protein_90 amidohydrolase -
SF83666_RS30915 96467..97368 - 902 Protein_91 substrate-binding domain-containing protein -
SF83666_RS30920 97412..97621 - 210 Protein_92 ABC transporter permease -
SF83666_RS30925 97621..98501 - 881 Protein_93 sugar ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB / gmd 1..742947 742947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(151-386)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 421 a.a.        Molecular weight: 45757.26 Da        Isoelectric Point: 4.8411

>T80455 WP_037436876.1 NZ_CP023072:c94055-92793 [Sinorhizobium fredii CCBAU 83666]
MTVRVLNVWWDGRIVGQFTQDRHGDIGFAYSEAWLDDENTLPLSASLPKRAEPFSRRECRPFFGGLLPEESQRLVTAQAL
GVSPANDFALLDRLGGDVAGALQLLPEDQEPIEAGSLPDQQPTPLDEAGIVRILDALPTRPLLAGQEGLRLSLAGAQSKV
PLVLIDGELALPVSGQATTHILKPPIARFPGTTENEAFVMRLAAAIGLDVAPVEPRSANGRPFLVVERYDRYRDADGVVH
RIHQEDFCQALGVPPETKYASEGGPTFKDCFELLRRVSARPATDVLKLLDAAIFNLVVGNADAHGKNFSILYDDQGPKMA
PLYDLLSTVAYPDLSPKMAMRIGKRVTLAEMDADGWQTFAKEAGVGLPLVRRRITNLVDSTAEAVARVLEDISDLYIDSA
RINHFADLVAGRAKLVRLSI

Download         Length: 1263 bp

>T80455 NZ_CP023072:c94055-92793 [Sinorhizobium fredii CCBAU 83666]
ATGACGGTAAGGGTTCTCAACGTCTGGTGGGATGGTCGCATTGTCGGGCAATTCACCCAGGACAGGCACGGTGATATCGG
CTTTGCCTACAGCGAAGCTTGGCTTGACGACGAAAACACGCTTCCGCTGTCTGCCTCCCTCCCGAAGCGCGCAGAACCTT
TTTCTCGTCGTGAATGCCGACCATTTTTCGGCGGCCTGTTGCCCGAGGAAAGCCAGCGTCTTGTGACAGCGCAGGCATTG
GGCGTTTCACCTGCGAACGACTTTGCGCTCCTTGATCGCTTGGGCGGCGACGTCGCCGGCGCACTTCAGCTTTTGCCGGA
AGATCAGGAGCCCATAGAGGCAGGGTCGCTTCCAGACCAGCAGCCGACACCTCTGGATGAGGCCGGAATCGTTCGGATAC
TGGACGCACTTCCGACGCGGCCGCTATTGGCCGGCCAGGAAGGCTTGAGGCTATCGCTAGCCGGCGCGCAGTCCAAGGTG
CCGCTTGTTTTGATTGACGGCGAGCTCGCGCTTCCAGTTTCCGGACAAGCAACAACACATATTCTGAAACCACCAATCGC
GCGCTTTCCAGGGACAACCGAAAACGAAGCATTCGTGATGAGACTCGCTGCAGCGATCGGCCTCGACGTCGCACCAGTCG
AACCGAGGTCTGCGAATGGGCGCCCTTTTCTTGTGGTCGAGCGATACGATCGCTATCGCGATGCCGACGGTGTGGTTCAC
CGCATCCACCAGGAAGACTTTTGCCAAGCACTTGGTGTGCCGCCTGAAACGAAATACGCCAGCGAGGGCGGGCCGACATT
CAAGGACTGCTTTGAGCTGTTGCGGCGAGTATCAGCGCGGCCCGCTACAGATGTGTTGAAGCTGCTGGACGCCGCGATCT
TCAACCTGGTTGTCGGAAATGCCGATGCCCACGGCAAGAATTTCTCGATCCTCTACGACGATCAGGGACCGAAAATGGCC
CCGCTGTACGATCTGCTTTCTACGGTCGCCTATCCAGACCTTTCACCGAAAATGGCCATGAGGATCGGGAAACGGGTCAC
GCTTGCCGAGATGGACGCTGATGGATGGCAGACTTTTGCAAAGGAAGCTGGAGTTGGCTTACCTTTGGTGCGCCGCCGAA
TTACAAACTTGGTCGACTCCACCGCTGAAGCCGTAGCGAGAGTATTGGAAGACATCTCAGACCTCTACATCGATTCAGCT
AGGATAAATCACTTTGCGGACTTGGTTGCAGGCCGCGCCAAACTCGTCAGGTTGTCGATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8204.72 Da        Isoelectric Point: 10.7650

>AT80455 WP_010875278.1 NZ_CP023072:c94282-94052 [Sinorhizobium fredii CCBAU 83666]
MAKRIKTPADIGALVRIVRKEQNLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSIDILAPGERRK

Download         Length: 231 bp

>AT80455 NZ_CP023072:c94282-94052 [Sinorhizobium fredii CCBAU 83666]
ATGGCGAAGCGCATCAAAACTCCAGCCGATATCGGCGCTCTAGTCCGCATCGTGCGCAAGGAGCAAAACTTGCGGCAGGA
TGAGCTTGCGGGCGTTGCCGGCGTTGGCCTCCGGTTCATTGTCGATCTCGAAGCGGGCAAACCGACAGCTCAGATAGGAA
AAGTGTTGCAGGTTTTGCAAACACTCGGCTGTTCGATCGATATTCTGGCTCCTGGTGAACGTAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249Q653

Antitoxin

Source ID Structure
AlphaFold DB A0A249Q849

References