Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 1969229..1969905 Replicon plasmid pSF83666b
Accession NZ_CP023071
Organism Sinorhizobium fredii CCBAU 83666

Toxin (Protein)


Gene name vapC Uniprot ID A0A249Q6Y3
Locus tag SF83666_RS28940 Protein ID WP_037434069.1
Coordinates 1969229..1969654 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A249Q515
Locus tag SF83666_RS28945 Protein ID WP_037395557.1
Coordinates 1969651..1969905 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF83666_RS35225 1964690..1965034 - 345 WP_153451912.1 hypothetical protein -
SF83666_RS28915 1965127..1965441 + 315 WP_037395542.1 PepSY domain-containing protein -
SF83666_RS28920 1965478..1966083 + 606 WP_037395543.1 hypothetical protein -
SF83666_RS28925 1966815..1967015 + 201 WP_037395547.1 hypothetical protein -
SF83666_RS28930 1967130..1967663 + 534 WP_037434071.1 DUF4334 domain-containing protein -
SF83666_RS28935 1968048..1968689 + 642 WP_037395551.1 AhpC/TSA family protein -
SF83666_RS28940 1969229..1969654 - 426 WP_037434069.1 type II toxin-antitoxin system VapC family toxin Toxin
SF83666_RS28945 1969651..1969905 - 255 WP_037395557.1 plasmid stabilization protein Antitoxin
SF83666_RS28950 1970472..1971437 + 966 WP_037434068.1 MBL fold metallo-hydrolase -
SF83666_RS28955 1971596..1972531 - 936 WP_014332647.1 LysR family transcriptional regulator -
SF83666_RS28960 1972656..1973453 + 798 WP_037434067.1 alpha/beta hydrolase -
SF83666_RS28965 1973517..1974518 + 1002 WP_037434066.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - kdsA / katA / pseB / htpB 1..2277056 2277056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15131.43 Da        Isoelectric Point: 6.2269

>T80454 WP_037434069.1 NZ_CP023071:c1969654-1969229 [Sinorhizobium fredii CCBAU 83666]
MIVLDTKVVSEAMEPFPNPVVHSWLNEQVAETLHLSSVTLAELMFGIGALPDGRRKKALSETLDGVLELFGDRVLPFDNG
AARHYAALAMKARAAGKGFPTPDGYIAAIAASKGFIIATRDVSPFEAAGLTVVNPWNNQRI

Download         Length: 426 bp

>T80454 NZ_CP023071:c1969654-1969229 [Sinorhizobium fredii CCBAU 83666]
ATGATCGTCTTGGATACGAAGGTCGTCTCCGAGGCGATGGAACCGTTTCCCAATCCGGTCGTGCACTCCTGGCTGAACGA
GCAGGTCGCCGAGACCCTACATCTGTCCAGTGTGACGTTGGCCGAACTGATGTTTGGAATCGGTGCATTGCCGGATGGCC
GGCGCAAGAAAGCTCTGAGCGAAACGCTTGACGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGACAACGGA
GCTGCCCGGCATTATGCCGCCTTAGCCATGAAGGCCCGAGCGGCCGGCAAGGGCTTCCCGACGCCCGATGGATATATTGC
CGCCATTGCCGCCTCCAAGGGATTTATCATAGCGACGCGTGATGTAAGCCCCTTTGAAGCGGCAGGACTTACCGTCGTAA
ATCCTTGGAATAATCAACGAATATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9512.92 Da        Isoelectric Point: 7.2068

>AT80454 WP_037395557.1 NZ_CP023071:c1969905-1969651 [Sinorhizobium fredii CCBAU 83666]
MAILTVRNVPDEVHRALRVRAAMHGRSTEAKVRDILESVVKPEQRVRMGDALAEVGRRLGLTNDDFAVFDEVRDKAPAEP
MRFE

Download         Length: 255 bp

>AT80454 NZ_CP023071:c1969905-1969651 [Sinorhizobium fredii CCBAU 83666]
ATGGCCATCCTGACAGTGAGGAATGTGCCCGATGAGGTGCATCGTGCTTTACGCGTGCGGGCGGCGATGCACGGCCGCAG
CACCGAGGCGAAAGTCCGTGACATTCTGGAAAGTGTGGTAAAGCCCGAGCAGCGCGTTCGCATGGGGGATGCCTTGGCTG
AGGTCGGCCGGCGGCTGGGACTGACCAATGATGATTTCGCAGTGTTTGATGAGGTACGTGACAAGGCGCCGGCGGAGCCA
ATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249Q6Y3

Antitoxin

Source ID Structure
AlphaFold DB A0A249Q515

References