Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 872477..873138 Replicon plasmid pSF83666b
Accession NZ_CP023071
Organism Sinorhizobium fredii CCBAU 83666

Toxin (Protein)


Gene name vapC Uniprot ID A0A249Q3X0
Locus tag SF83666_RS23760 Protein ID WP_037436122.1
Coordinates 872477..872914 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A249Q231
Locus tag SF83666_RS23765 Protein ID WP_037436124.1
Coordinates 872911..873138 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF83666_RS23730 868065..869315 - 1251 WP_037436116.1 bifunctional alpha/beta hydrolase/OsmC family protein -
SF83666_RS23735 869527..870033 - 507 WP_037436117.1 peptide-methionine (S)-S-oxide reductase MsrA -
SF83666_RS23740 870034..870477 - 444 WP_037436118.1 peptide-methionine (R)-S-oxide reductase MsrB -
SF83666_RS23745 870767..871222 - 456 WP_014332040.1 carboxymuconolactone decarboxylase family protein -
SF83666_RS23750 871247..871663 - 417 WP_037436119.1 cupin domain-containing protein -
SF83666_RS23755 871904..872437 + 534 WP_037436120.1 Rrf2 family transcriptional regulator -
SF83666_RS23760 872477..872914 - 438 WP_037436122.1 PIN domain-containing protein Toxin
SF83666_RS23765 872911..873138 - 228 WP_037436124.1 hypothetical protein Antitoxin
SF83666_RS23770 873579..874535 + 957 WP_037436125.1 patatin-like phospholipase family protein -
SF83666_RS23775 875050..876441 - 1392 WP_037436126.1 S8 family serine peptidase -
SF83666_RS23780 876584..877126 + 543 WP_037436127.1 RNA polymerase sigma factor -
SF83666_RS23785 877123..877905 + 783 WP_060564129.1 anti-sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - kdsA / katA / pseB / htpB 1..2277056 2277056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15277.51 Da        Isoelectric Point: 6.7480

>T80453 WP_037436122.1 NZ_CP023071:c872914-872477 [Sinorhizobium fredii CCBAU 83666]
VTFLLDVNVLIALIDPGHVAHDDAHDWFASTGKTAWATCPITENGVLRIVGNPKYPNSPGSPALVMEIVGKLRSLPGHCF
WPDDVSLVGSGDIAPAKILTSGQVTDTYLLALAKAHDGQLATFDRKLTAAAVTRGNSALHLITGK

Download         Length: 438 bp

>T80453 NZ_CP023071:c872914-872477 [Sinorhizobium fredii CCBAU 83666]
GTGACCTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGGGCCATGTAGCCCATGATGATGCGCACGATTG
GTTCGCATCGACCGGCAAGACCGCGTGGGCGACCTGTCCCATTACCGAAAACGGTGTCCTCCGCATTGTCGGCAATCCCA
AATACCCCAATTCTCCCGGCTCACCGGCACTCGTGATGGAGATCGTTGGCAAGTTGCGGTCGCTCCCTGGTCATTGTTTT
TGGCCGGACGACGTGAGCCTCGTCGGGTCAGGTGATATCGCTCCCGCGAAAATCCTGACTTCGGGGCAGGTAACCGATAC
CTATCTGCTTGCGCTCGCGAAGGCGCATGACGGCCAGTTGGCGACATTCGATCGCAAGCTAACAGCAGCGGCGGTCACGA
GGGGCAACTCTGCCTTGCACCTGATCACCGGCAAATAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8213.43 Da        Isoelectric Point: 9.1311

>AT80453 WP_037436124.1 NZ_CP023071:c873138-872911 [Sinorhizobium fredii CCBAU 83666]
MRTTLAIDDDVLIAAKAMAAQQHRSLGEVISELARRSLRRPRSGGERNGIPLLSSRPDTPPVTLEIVNALRDELP

Download         Length: 228 bp

>AT80453 NZ_CP023071:c873138-872911 [Sinorhizobium fredii CCBAU 83666]
ATGAGAACGACACTAGCGATCGATGACGATGTCCTGATCGCCGCCAAGGCGATGGCAGCCCAGCAGCACCGCAGTCTCGG
CGAGGTCATTTCCGAGCTGGCGAGACGTTCTTTGCGCCGGCCGCGCAGTGGTGGCGAGCGCAATGGAATTCCTCTTCTGT
CGTCGCGACCCGATACCCCGCCAGTTACCCTCGAGATCGTCAACGCCTTGCGCGACGAACTGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249Q3X0

Antitoxin

Source ID Structure
AlphaFold DB A0A249Q231

References