Detailed information of TA system TA080452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 805322..806257
• Replicon: plasmid pSF83666b
• Accession: NZ_CP023071
• Organism: Sinorhizobium fredii CCBAU 83666

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: SF83666_RS23430
• Protein ID: WP_037431985.1
• Coordinates: 805697..806257 (+)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A249Q1W0
• Locus tag: SF83666_RS23425
• Protein ID: WP_037431986.1
• Coordinates: 805322..805693 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF83666_RS23405	801199..801582	+	384	WP_037431990.1	RidA family protein	-
SF83666_RS23410	801598..803586	+	1989	WP_037431989.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
SF83666_RS23415	803583..804452	+	870	WP_037431988.1	hydroxymethylglutaryl-CoA lyase	-
SF83666_RS23420	804449..805234	+	786	WP_037435553.1	crotonase/enoyl-CoA hydratase family protein	-
SF83666_RS23425	805322..805693	+	372	WP_037431986.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
SF83666_RS23430	805697..806257	+	561	WP_037431985.1	RES family NAD+ phosphorylase	Toxin
SF83666_RS23435	806270..807634	-	1365	WP_037431984.1	FAD-binding oxidoreductase	-
SF83666_RS23440	807768..808874	-	1107	WP_037435550.1	sulfate/molybdate ABC transporter ATP-binding protein	-
SF83666_RS23445	808890..809777	-	888	WP_037431979.1	sulfate ABC transporter permease subunit CysW	-
SF83666_RS23450	809767..810624	-	858	WP_037431978.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	kdsA / katA / pseB / htpB	1..2277056	2277056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-179)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20775.69 Da
• Isoelectric Point: 6.9699

>T80452 WP_037431985.1 NZ_CP023071:805697-806257 [Sinorhizobium fredii CCBAU 83666]
MKKSLSGPSSSQPDLDVARMAEPMTLWRAFVPRWAHMPLSGDGAARFGGRWNPVGMPTIYAARELSTAWAEYNQGFVQHP
ALIVQLELRDAKLADLTDGDVLAELGLDEAIHRCEWRDELDKGLVPQTHKTHTDLLKRGYDGVIYPSYMSPGGTCVALWR
WNGRGRPRLDVIDPEGRLPKSPASWL

Nucleotide

• Length: 561 bp

>T80452 NZ_CP023071:805697-806257 [Sinorhizobium fredii CCBAU 83666]
ATGAAGAAAAGTCTGAGCGGCCCGTCCTCCTCCCAGCCCGACTTGGACGTGGCGAGGATGGCCGAGCCGATGACGCTCTG
GCGCGCCTTTGTCCCGCGCTGGGCCCATATGCCGCTCTCCGGCGACGGGGCCGCCCGCTTTGGTGGCCGGTGGAATCCGG
TCGGCATGCCGACGATCTACGCGGCGCGTGAACTTTCCACCGCCTGGGCGGAGTATAATCAGGGCTTCGTCCAGCACCCG
GCGCTGATCGTGCAACTCGAGCTTCGCGACGCAAAACTGGCCGATCTCACGGATGGCGATGTTCTCGCCGAGTTGGGACT
AGACGAGGCAATCCATCGCTGCGAATGGCGCGACGAACTTGACAAGGGCTTGGTCCCGCAGACGCATAAGACCCACACCG
ATCTTTTGAAGCGCGGCTACGACGGCGTGATCTACCCCTCCTACATGTCGCCCGGAGGCACCTGCGTCGCCCTCTGGCGC
TGGAATGGACGGGGCAGGCCGCGGCTCGACGTCATCGATCCGGAAGGTCGGCTGCCGAAGTCGCCCGCATCGTGGCTTTA
G

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13141.25 Da
• Isoelectric Point: 9.8798

>AT80452 WP_037431986.1 NZ_CP023071:805322-805693 [Sinorhizobium fredii CCBAU 83666]
MIAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELSKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAYDLVGEGKADKVLAYLESIRAGVYA

Nucleotide

• Length: 372 bp

>AT80452 NZ_CP023071:805322-805693 [Sinorhizobium fredii CCBAU 83666]
ATGATAGCTGTCGATTTCCAGATTCCGGCGGCGCGCTTCGGAGACGACCATTCTCCGTTCCTGTCGGCGCGCCTTGTCGC
CGATCGGCTGGGCGTCACGCTTGCCGAACTTTCCAAGCTGATTGGCGTCGCGCGCAACACGCTGACGGCAAAGTCCGGCG
CGCGAAAGGTCGACAGCGCCTTGAGCAAGGTCGTTCGCATCCTTGCCATGGCCTCCGAGATGGCCGGCGACGAGGCGCGT
GCCGTCATCTGGTTCAAGCATCAGCCGATTCCCGGCTGGGCCGGCAAGACCGCATATGATCTGGTCGGCGAAGGCAAGGC
CGACAAGGTTCTTGCCTATCTCGAGTCGATCCGCGCCGGCGTCTACGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A249Q1W0