Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 586511..587085 Replicon plasmid pSF83666b
Accession NZ_CP023071
Organism Sinorhizobium fredii CCBAU 83666

Toxin (Protein)


Gene name vapC Uniprot ID A0A844A9Q1
Locus tag SF83666_RS22410 Protein ID WP_037436311.1
Coordinates 586511..586894 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2Z3G9D2
Locus tag SF83666_RS22415 Protein ID WP_037402653.1
Coordinates 586891..587085 (-) Length 65 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF83666_RS22385 581966..582625 - 660 WP_037435970.1 ABC transporter ATP-binding protein -
SF83666_RS22390 582622..583392 - 771 WP_037435971.1 ABC transporter permease -
SF83666_RS22395 583397..584698 - 1302 WP_060564044.1 capsular polysaccharide biosynthesis protein -
SF83666_RS22400 584805..585719 - 915 WP_095689776.1 nucleotidyltransferase and HEPN domain-containing protein -
SF83666_RS22410 586511..586894 - 384 WP_037436311.1 type II toxin-antitoxin system VapC family toxin Toxin
SF83666_RS22415 586891..587085 - 195 WP_037402653.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SF83666_RS35060 587325..587747 - 423 WP_141322358.1 hypothetical protein -
SF83666_RS22425 587783..588949 - 1167 WP_037436316.1 ATP-binding protein -
SF83666_RS35800 589319..589954 - 636 WP_210205944.1 calcium-binding protein -
SF83666_RS35805 590117..590452 - 336 Protein_570 calcium-binding protein -
SF83666_RS35810 590846..591115 - 270 Protein_571 calcium-binding protein -
SF83666_RS35815 591089..591358 - 270 Protein_572 calcium-binding protein -
SF83666_RS35820 591596..591901 - 306 Protein_573 calcium-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - kdsA / katA / pseB / htpB 1..2277056 2277056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14159.34 Da        Isoelectric Point: 6.6403

>T80451 WP_037436311.1 NZ_CP023071:c586894-586511 [Sinorhizobium fredii CCBAU 83666]
VRLADTGIWIDHFRQADPQLIQTIKNDMLLCHPAILGELALGSLRNREVTLGFLAVQREVTVATHDEVMAMIDEHQLYSM
GIGYTDAHLLASTLIDSRAELWTRDKRLVRAAKKAGALVVSDVTRPN

Download         Length: 384 bp

>T80451 NZ_CP023071:c586894-586511 [Sinorhizobium fredii CCBAU 83666]
GTGAGGCTAGCCGATACTGGAATCTGGATCGACCACTTCCGTCAAGCTGACCCACAACTAATCCAAACGATAAAAAACGA
TATGCTGCTTTGCCATCCGGCCATACTTGGCGAGCTTGCCTTGGGAAGCCTGCGCAACCGTGAGGTTACGCTCGGGTTCT
TGGCGGTCCAGCGGGAGGTCACTGTCGCTACGCATGATGAAGTGATGGCAATGATCGATGAACACCAACTTTACAGCATG
GGTATAGGCTACACGGACGCCCACCTGCTCGCGTCAACTCTCATCGACAGCCGCGCTGAGTTGTGGACACGAGACAAGCG
ACTGGTCAGGGCCGCGAAAAAGGCAGGCGCGCTTGTTGTTTCCGACGTCACTAGGCCCAACTAG

Antitoxin


Download         Length: 65 a.a.        Molecular weight: 6879.98 Da        Isoelectric Point: 10.5929

>AT80451 WP_037402653.1 NZ_CP023071:c587085-586891 [Sinorhizobium fredii CCBAU 83666]
MRSTINIDDSLMEKAKALTGTKETAAVVRQALETLVRVEAGKRLVALGGTMPDAEAGPRRRAEK

Download         Length: 195 bp

>AT80451 NZ_CP023071:c587085-586891 [Sinorhizobium fredii CCBAU 83666]
ATGCGGTCAACCATCAATATAGACGATAGCCTGATGGAAAAGGCGAAGGCCCTGACGGGCACGAAGGAAACGGCGGCGGT
CGTTCGGCAAGCACTGGAAACGTTGGTCCGGGTGGAAGCAGGCAAGCGTCTCGTTGCCTTGGGTGGCACTATGCCCGACG
CCGAGGCTGGCCCGCGTAGGCGGGCCGAAAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A844A9Q1

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3G9D2

References