Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 163705..165184 Replicon plasmid pSF83666b
Accession NZ_CP023071
Organism Sinorhizobium fredii CCBAU 83666

Toxin (Protein)


Gene name rlegT Uniprot ID A0A249Q0K5
Locus tag SF83666_RS20295 Protein ID WP_037435306.1
Coordinates 164315..165184 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A844AJL2
Locus tag SF83666_RS20290 Protein ID WP_037435305.1
Coordinates 163705..164322 (+) Length 206 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF83666_RS20260 158735..159202 + 468 WP_037435294.1 MarR family transcriptional regulator -
SF83666_RS20265 159217..159996 - 780 WP_037385612.1 SDR family oxidoreductase -
SF83666_RS20270 159996..160316 - 321 WP_014531263.1 nuclear transport factor 2 family protein -
SF83666_RS20275 160412..161317 + 906 WP_037435299.1 LysR family transcriptional regulator -
SF83666_RS20280 161818..162327 + 510 WP_037435301.1 nucleoside kinase -
SF83666_RS20285 162647..163273 + 627 WP_037435303.1 class I SAM-dependent methyltransferase -
SF83666_RS20290 163705..164322 + 618 WP_037435305.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
SF83666_RS20295 164315..165184 + 870 WP_037435306.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
SF83666_RS20300 165181..166148 + 968 Protein_158 3'-5' exonuclease -
SF83666_RS20305 166093..166642 + 550 Protein_159 hypothetical protein -
SF83666_RS20310 166629..167978 - 1350 WP_037435307.1 hypothetical protein -
SF83666_RS20315 168521..168904 - 384 WP_050984148.1 pyridoxal-phosphate dependent enzyme -
SF83666_RS20320 169052..169261 + 210 WP_037400647.1 CsbD family protein -
SF83666_RS35795 169314..169916 - 603 WP_037435309.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - kdsA / katA / pseB / htpB 1..2277056 2277056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-258)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(9-56)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32422.16 Da        Isoelectric Point: 5.7865

>T80450 WP_037435306.1 NZ_CP023071:164315-165184 [Sinorhizobium fredii CCBAU 83666]
MAKEIKNPGASVRARLLQLAKTSGQSFDLVLARFALERLLFRLSRSPHAGRFVLKGAMLMMSWFDDPHRSTRDLDLLGFG
NPEADQMLATFREILAQQADDGVTFDADTLRVDRIREELEYGGLRLRVVASISGARINLTIDIGFGDALEPGTEMLDYPS
MLDFPAPRLRAYARETVIAEKFEAMVMLGRANSRMKDFYDIWILSRSCDFSDDRLGRAIAATFERRETPIPNDLPDALTD
AFARDEQKQRQWRAFVEGVAHSPGVLTDIIPEIATFLMPHAAAAARLSK

Download         Length: 870 bp

>T80450 NZ_CP023071:164315-165184 [Sinorhizobium fredii CCBAU 83666]
ATGGCTAAGGAAATCAAAAATCCTGGCGCCTCGGTGCGAGCCCGCCTGTTGCAACTCGCCAAGACAAGCGGGCAAAGCTT
CGATCTGGTTCTGGCGCGTTTTGCCCTTGAGCGGTTGCTGTTCCGGCTCAGCCGGTCGCCCCATGCCGGTCGCTTCGTGC
TGAAGGGCGCCATGCTCATGATGAGCTGGTTCGACGACCCGCACCGCAGCACGCGCGACCTTGACCTTTTGGGCTTTGGC
AACCCCGAAGCGGACCAAATGCTGGCGACCTTTCGGGAAATTCTGGCGCAACAGGCTGATGACGGTGTCACGTTCGACGC
CGACACCTTGCGTGTAGATCGCATCCGCGAAGAACTAGAATATGGTGGGCTAAGGCTGCGGGTGGTTGCCTCGATCAGCG
GCGCTCGAATCAACCTGACGATCGACATCGGCTTTGGCGATGCGCTCGAGCCCGGGACGGAGATGTTAGACTATCCTTCC
ATGCTGGATTTCCCAGCGCCTCGGCTCCGAGCCTATGCGCGAGAGACGGTCATTGCGGAGAAGTTCGAGGCGATGGTTAT
GCTGGGTCGTGCAAATAGTCGGATGAAGGATTTCTACGACATTTGGATTCTCAGCAGGTCCTGTGATTTCAGCGATGACC
GCCTGGGGCGTGCAATCGCCGCCACCTTCGAGCGGCGCGAGACACCGATCCCCAACGACCTTCCAGATGCCTTGACGGAC
GCCTTTGCCCGGGACGAGCAAAAGCAGCGGCAATGGCGTGCTTTCGTTGAAGGCGTTGCGCACAGTCCGGGGGTTCTGAC
GGACATTATTCCGGAGATTGCTACATTCCTGATGCCGCATGCAGCCGCAGCCGCGAGGCTGAGCAAATGA

Antitoxin


Download         Length: 206 a.a.        Molecular weight: 22328.95 Da        Isoelectric Point: 10.1307

>AT80450 WP_037435305.1 NZ_CP023071:163705-164322 [Sinorhizobium fredii CCBAU 83666]
MLGSVTQRQIARIVLIERGIARLVELRNAGVTAATMSRMERDGEVLRLARGLYQLPDAELDANHSLAETAKRVPKAVVCL
VSALVFHGLTDQLPRYVWLAIGRKDWAPKPDGTPIRIVRFAESLLHDGVETHLVEGVPVKVFGIAKTIADCFRHRSKIGL
SVAIEGLQEALRQRKATPGDIASQAERGGVGTVIRPYLEALTANG

Download         Length: 618 bp

>AT80450 NZ_CP023071:163705-164322 [Sinorhizobium fredii CCBAU 83666]
ATGCTTGGCTCCGTCACCCAACGTCAAATCGCCCGGATCGTCCTCATTGAGCGCGGCATAGCGCGTTTGGTGGAACTTCG
GAACGCAGGTGTGACAGCAGCAACCATGAGCCGGATGGAACGGGATGGCGAGGTGCTGCGGCTTGCTCGTGGTCTCTATC
AACTTCCCGATGCCGAGCTCGATGCTAACCATAGTCTGGCAGAGACTGCCAAGCGTGTTCCGAAAGCCGTTGTCTGCCTT
GTTTCAGCCCTCGTATTCCACGGCCTAACCGATCAGCTGCCGAGATACGTGTGGCTTGCCATTGGCCGCAAGGATTGGGC
TCCGAAACCAGATGGCACACCTATTCGTATCGTGCGCTTTGCCGAGAGCCTGCTCCACGATGGCGTGGAAACTCATCTGG
TCGAAGGTGTCCCTGTAAAGGTCTTTGGGATCGCCAAAACAATTGCCGACTGCTTCCGCCATCGCAGCAAGATCGGTCTT
TCTGTGGCGATCGAAGGACTTCAGGAAGCACTGCGGCAGCGCAAGGCCACGCCCGGCGACATTGCCAGCCAGGCCGAACG
CGGTGGTGTTGGCACCGTGATCCGCCCCTATCTCGAGGCGCTGACCGCCAATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249Q0K5

Antitoxin

Source ID Structure
AlphaFold DB A0A844AJL2

References