Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/VapC(toxin) |
| Location | 138771..139411 | Replicon | plasmid pSF83666b |
| Accession | NZ_CP023071 | ||
| Organism | Sinorhizobium fredii CCBAU 83666 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | A0A249Q0D6 |
| Locus tag | SF83666_RS20165 | Protein ID | WP_037435265.1 |
| Coordinates | 138771..139157 (-) | Length | 129 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | A0A249Q008 |
| Locus tag | SF83666_RS20170 | Protein ID | WP_037435268.1 |
| Coordinates | 139157..139411 (-) | Length | 85 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SF83666_RS20120 | 133866..134777 | + | 912 | Protein_122 | nucleotidyltransferase and HEPN domain-containing protein | - |
| SF83666_RS20125 | 135096..135439 | - | 344 | Protein_123 | hypothetical protein | - |
| SF83666_RS20130 | 135450..135827 | - | 378 | WP_037435254.1 | type II toxin-antitoxin system VapC family toxin | - |
| SF83666_RS20135 | 135824..136114 | - | 291 | WP_037435255.1 | type II toxin-antitoxin system prevent-host-death family antitoxin | - |
| SF83666_RS20140 | 136344..136798 | + | 455 | Protein_126 | replication initiation protein RepC | - |
| SF83666_RS20145 | 137057..137266 | + | 210 | WP_037435258.1 | cold-shock protein | - |
| SF83666_RS20155 | 137921..138501 | - | 581 | Protein_128 | tyrosine-type recombinase/integrase | - |
| SF83666_RS20160 | 138445..138718 | + | 274 | Protein_129 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| SF83666_RS20165 | 138771..139157 | - | 387 | WP_037435265.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| SF83666_RS20170 | 139157..139411 | - | 255 | WP_037435268.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| SF83666_RS35010 | 139692..139892 | - | 201 | WP_050994744.1 | hypothetical protein | - |
| SF83666_RS20175 | 139818..140542 | + | 725 | Protein_133 | DUF1612 domain-containing protein | - |
| SF83666_RS20180 | 140579..141532 | - | 954 | WP_037435270.1 | ornithine cyclodeaminase family protein | - |
| SF83666_RS20185 | 141751..142548 | - | 798 | WP_037435963.1 | M55 family metallopeptidase | - |
| SF83666_RS20190 | 142712..143599 | - | 888 | WP_037435277.1 | MurR/RpiR family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | kdsA / katA / pseB / htpB | 1..2277056 | 2277056 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 129 a.a. Molecular weight: 14015.87 Da Isoelectric Point: 4.5256
>T80449 WP_037435265.1 NZ_CP023071:c139157-138771 [Sinorhizobium fredii CCBAU 83666]
MVIDTSAIVAIAFNEPEAEDYERKIVDAPRRFMSAATVLELAIVIEARLGEAGAAELDLWLHKAGVEIVAVDADQIAVAR
RAWRSYGKGRHPAGLNYGDCFSYALARTRNEPLLYKGDDFSCTDIEAA
MVIDTSAIVAIAFNEPEAEDYERKIVDAPRRFMSAATVLELAIVIEARLGEAGAAELDLWLHKAGVEIVAVDADQIAVAR
RAWRSYGKGRHPAGLNYGDCFSYALARTRNEPLLYKGDDFSCTDIEAA
Download Length: 387 bp
>T80449 NZ_CP023071:c139157-138771 [Sinorhizobium fredii CCBAU 83666]
ATGGTCATCGACACATCCGCCATCGTCGCGATCGCGTTCAATGAGCCGGAAGCGGAAGACTACGAGCGAAAAATCGTCGA
CGCTCCCCGACGTTTCATGTCGGCCGCGACAGTCCTCGAGCTCGCCATCGTCATCGAGGCGCGCCTCGGCGAAGCCGGCG
CCGCAGAACTCGATCTCTGGCTTCACAAGGCGGGCGTCGAAATCGTCGCCGTCGACGCGGATCAGATCGCCGTCGCAAGG
CGAGCCTGGCGCAGCTACGGCAAGGGCCGACATCCGGCAGGTCTGAACTATGGCGACTGTTTTTCCTATGCGCTGGCCAG
GACGCGCAACGAACCTCTGCTCTACAAAGGCGATGATTTCTCGTGCACGGACATCGAGGCGGCATGA
ATGGTCATCGACACATCCGCCATCGTCGCGATCGCGTTCAATGAGCCGGAAGCGGAAGACTACGAGCGAAAAATCGTCGA
CGCTCCCCGACGTTTCATGTCGGCCGCGACAGTCCTCGAGCTCGCCATCGTCATCGAGGCGCGCCTCGGCGAAGCCGGCG
CCGCAGAACTCGATCTCTGGCTTCACAAGGCGGGCGTCGAAATCGTCGCCGTCGACGCGGATCAGATCGCCGTCGCAAGG
CGAGCCTGGCGCAGCTACGGCAAGGGCCGACATCCGGCAGGTCTGAACTATGGCGACTGTTTTTCCTATGCGCTGGCCAG
GACGCGCAACGAACCTCTGCTCTACAAAGGCGATGATTTCTCGTGCACGGACATCGAGGCGGCATGA
Antitoxin
Download Length: 85 a.a. Molecular weight: 9475.81 Da Isoelectric Point: 6.3783
>AT80449 WP_037435268.1 NZ_CP023071:c139411-139157 [Sinorhizobium fredii CCBAU 83666]
MALSIKDIETERLAKALAEKTGETITMATRRALEERLRRLANVGNRDVLLEELGASRRRWAKLPVLDNRSADEILGYDEN
GLPN
MALSIKDIETERLAKALAEKTGETITMATRRALEERLRRLANVGNRDVLLEELGASRRRWAKLPVLDNRSADEILGYDEN
GLPN
Download Length: 255 bp
>AT80449 NZ_CP023071:c139411-139157 [Sinorhizobium fredii CCBAU 83666]
ATGGCGCTCAGCATCAAAGATATAGAGACGGAACGCCTTGCAAAGGCACTGGCCGAGAAAACCGGTGAAACCATTACCAT
GGCGACCCGCCGGGCACTGGAGGAACGGCTGCGGCGCCTCGCAAACGTGGGAAATCGCGATGTTCTTCTCGAGGAGCTTG
GCGCCAGCCGCAGACGCTGGGCGAAGCTGCCGGTGCTCGACAACCGCTCCGCCGACGAGATCTTAGGGTATGACGAAAAC
GGGCTCCCCAACTGA
ATGGCGCTCAGCATCAAAGATATAGAGACGGAACGCCTTGCAAAGGCACTGGCCGAGAAAACCGGTGAAACCATTACCAT
GGCGACCCGCCGGGCACTGGAGGAACGGCTGCGGCGCCTCGCAAACGTGGGAAATCGCGATGTTCTTCTCGAGGAGCTTG
GCGCCAGCCGCAGACGCTGGGCGAAGCTGCCGGTGCTCGACAACCGCTCCGCCGACGAGATCTTAGGGTATGACGAAAAC
GGGCTCCCCAACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A249Q0D6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A249Q008 |