Detailed information of TA system TA080448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 130318..130966
• Replicon: chromosome
• Accession: NZ_CP023070
• Organism: Sinorhizobium fredii CCBAU 83666

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G9AA33
• Locus tag: SF83666_RS00620
• Protein ID: WP_014327150.1
• Coordinates: 130712..130966 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SF83666_RS00615
• Protein ID: WP_037391843.1
• Coordinates: 130318..130698 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF83666_RS00600	128034..128699	+	666	WP_014327146.1	CDP-alcohol phosphatidyltransferase family protein	-
SF83666_RS00605	128696..129307	+	612	WP_014327147.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
SF83666_RS00610	129315..130256	+	942	WP_014327148.1	phosphatidate cytidylyltransferase	-
SF83666_RS00615	130318..130698	-	381	WP_037391843.1	helix-turn-helix transcriptional regulator	Antitoxin
SF83666_RS00620	130712..130966	-	255	WP_014327150.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SF83666_RS00630	131344..132354	-	1011	WP_014327192.1	nitronate monooxygenase family protein	-
SF83666_RS00635	132639..133811	+	1173	WP_037391917.1	hypothetical protein	-
SF83666_RS00640	133827..134729	-	903	WP_037391890.1	polyphosphate kinase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-125)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9407.94 Da
• Isoelectric Point: 10.8582

>T80448 WP_014327150.1 NZ_CP023070:c130966-130712 [Sinorhizobium fredii CCBAU 83666]
MKTIRYTREALKTLTQIPANVAKRIRSKIEQYASDPASLANNIKALKGLQGTHRLRVGDWRVIFTEDGEIIAIKKIAPRG
GAYD

Nucleotide

• Length: 255 bp

>T80448 NZ_CP023070:c130966-130712 [Sinorhizobium fredii CCBAU 83666]
ATGAAAACGATCCGGTACACCCGTGAAGCGCTGAAAACCTTGACACAAATTCCCGCGAACGTTGCCAAGCGGATCCGCTC
GAAAATCGAGCAATATGCATCGGACCCGGCAAGTCTCGCTAACAACATCAAGGCACTGAAAGGGTTGCAAGGTACACATC
GGCTTCGGGTCGGAGACTGGCGCGTTATCTTTACGGAAGACGGCGAAATCATCGCCATCAAGAAGATCGCCCCGCGCGGC
GGCGCATACGACTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14006.05 Da
• Isoelectric Point: 4.4452

>AT80448 WP_037391843.1 NZ_CP023070:c130698-130318 [Sinorhizobium fredii CCBAU 83666]
MVLQFITTPAGDRMVVMAEDEYKMLLHAAEAAEDAEDVADVKRINERLARGEEELIPADIVNRILDGESKVKVWREYRKL
TLRELAAKVDISPAYLSQIEGGSRDGTFETMKKIAAALGITVDELA

Nucleotide

• Length: 381 bp

>AT80448 NZ_CP023070:c130698-130318 [Sinorhizobium fredii CCBAU 83666]
ATGGTACTACAGTTCATCACTACCCCGGCCGGCGACCGAATGGTCGTCATGGCCGAGGACGAATACAAGATGCTTTTGCA
TGCCGCAGAGGCTGCCGAGGACGCCGAAGACGTCGCGGACGTAAAGCGGATCAATGAGCGCCTAGCTCGCGGCGAAGAAG
AGTTGATCCCCGCCGATATCGTCAACCGCATCCTTGATGGCGAAAGCAAGGTGAAGGTCTGGCGCGAGTACAGGAAACTA
ACCCTCCGAGAACTCGCGGCCAAGGTCGATATCAGCCCGGCGTACCTTTCTCAAATCGAGGGCGGCAGCCGCGACGGCAC
CTTTGAGACGATGAAGAAGATAGCGGCCGCTCTCGGTATCACGGTGGACGAACTCGCATAA

Structures

Toxin

• AlphaFold DB: G9AA33