Detailed information of TA system TA080444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 516714..517594
• Replicon: plasmid pSJ05684b
• Accession: NZ_CP023068
• Organism: Ensifer sojae CCBAU 05684

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: SJ05684_RS20065
• Protein ID: WP_034854386.1
• Coordinates: 517103..517594 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A249PJV5
• Locus tag: SJ05684_RS20060
• Protein ID: WP_034854360.1
• Coordinates: 516714..517085 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SJ05684_RS20040	512599..512982	+	384	WP_034854364.1	RidA family protein	-
SJ05684_RS20045	512999..514987	+	1989	WP_034854363.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
SJ05684_RS20050	514984..515853	+	870	WP_034854362.1	hydroxymethylglutaryl-CoA lyase	-
SJ05684_RS20055	515850..516635	+	786	WP_034854361.1	crotonase/enoyl-CoA hydratase family protein	-
SJ05684_RS20060	516714..517085	+	372	WP_034854360.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
SJ05684_RS20065	517103..517594	+	492	WP_034854386.1	RES domain-containing protein	Toxin
SJ05684_RS20070	517746..518789	-	1044	WP_095694328.1	sulfate/molybdate ABC transporter ATP-binding protein	-
SJ05684_RS20075	518850..519875	-	1026	WP_034859896.1	sulfate ABC transporter substrate-binding protein	-
SJ05684_RS20080	520255..521364	-	1110	WP_095694329.1	sulfate/molybdate ABC transporter ATP-binding protein	-
SJ05684_RS20085	521380..522267	-	888	WP_034855452.1	sulfate ABC transporter permease subunit CysW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..2011513	2011513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-156)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18100.65 Da
• Isoelectric Point: 7.4788

>T80444 WP_034854386.1 NZ_CP023068:517103-517594 [Ensifer sojae CCBAU 05684]
MALWRAFVPRWAHMPLSGEGAARFGGRWNPVGTPTIYAARELSTAWAEYNQGFVQHPALIVKLELRDAKLADLTDAHVLA
ELGLEDTIHRCEWREELDRGVAPQTHTVHANLVARGFHGVIYPSFMSPGGTCAALWRWNGKNGPKLDVVDPEGRLPKSPA
SWI

Nucleotide

• Length: 492 bp

>T80444 NZ_CP023068:517103-517594 [Ensifer sojae CCBAU 05684]
ATGGCGCTCTGGCGCGCCTTCGTGCCGCGCTGGGCCCACATGCCCCTCTCCGGCGAAGGTGCCGCGCGCTTCGGCGGCCG
ATGGAACCCGGTCGGCACGCCGACGATCTATGCGGCGCGCGAGCTTTCCACCGCCTGGGCGGAATACAACCAGGGTTTCG
TCCAGCATCCGGCGCTTATCGTGAAGCTGGAACTGCGTGACGCGAAACTCGCGGACCTGACCGACGCCCACGTGCTTGCT
GAGCTTGGCCTCGAGGACACCATTCACCGCTGCGAATGGCGCGAGGAACTTGACCGGGGAGTGGCGCCGCAGACGCACAC
CGTGCACGCGAACCTCGTCGCCCGTGGGTTTCACGGCGTGATCTACCCATCCTTCATGTCACCCGGCGGCACCTGTGCCG
CGCTCTGGCGCTGGAATGGCAAGAATGGACCGAAACTCGACGTCGTCGATCCCGAGGGCCGGCTGCCAAAGTCGCCGGCG
TCGTGGATTTAG

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13237.25 Da
• Isoelectric Point: 8.4578

>AT80444 WP_034854360.1 NZ_CP023068:516714-517085 [Ensifer sojae CCBAU 05684]
MIAVDFDITAARFGEDHSQYLSARLVADRLGVTLAELAKLIGVARNTLTAKSGVRKVDSALSQVVRILAMASEMAGDETR
AVIWFKHQPIPGWAGKTAYDLVGEGKADKVLAYLESVRAGVYS

Nucleotide

• Length: 372 bp

>AT80444 NZ_CP023068:516714-517085 [Ensifer sojae CCBAU 05684]
ATGATCGCTGTCGATTTCGACATTACCGCTGCGCGCTTCGGAGAGGACCACTCGCAGTATCTGTCGGCCCGCCTGGTTGC
CGACCGGCTGGGCGTGACGCTCGCGGAACTCGCCAAGCTGATCGGCGTCGCCCGCAACACCTTGACCGCCAAGTCCGGGG
TGCGGAAGGTCGACAGCGCGCTGAGCCAGGTCGTGCGCATCCTTGCAATGGCATCGGAAATGGCGGGGGACGAGACCCGT
GCCGTCATCTGGTTCAAGCATCAGCCGATCCCGGGCTGGGCCGGGAAGACGGCCTATGACCTGGTGGGCGAAGGCAAGGC
CGATAAGGTGCTTGCCTATCTCGAGTCCGTGCGCGCCGGCGTCTATTCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A249PJV5