Detailed information of TA system TA080441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1366910..1367577
• Replicon: plasmid pSS05631b
• Accession: NZ_CP023064
• Organism: Sinorhizobium sp. CCBAU 05631

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SS05631_RS25845
• Protein ID: WP_037415845.1
• Coordinates: 1367134..1367577 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2A6M9B6
• Locus tag: SS05631_RS25840
• Protein ID: WP_037415848.1
• Coordinates: 1366910..1367137 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS05631_RS25820	1362896..1363789	+	894	WP_037415861.1	intradiol ring-cleavage dioxygenase	-
SS05631_RS25825	1363814..1364596	+	783	WP_037415858.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
SS05631_RS25830	1364605..1365396	+	792	WP_037415855.1	4-hydroxy-2-oxoheptanedioate aldolase	-
SS05631_RS25835	1365383..1366066	+	684	WP_037415852.1	transporter substrate-binding domain-containing protein	-
SS05631_RS25840	1366910..1367137	+	228	WP_037415848.1	hypothetical protein	Antitoxin
SS05631_RS25845	1367134..1367577	+	444	WP_037415845.1	PIN domain-containing protein	Toxin
SS05631_RS25855	1369059..1369394	-	336	Protein_1240	hypothetical protein	-
SS05631_RS25860	1369612..1371006	-	1395	WP_037415843.1	HAMP domain-containing protein	-
SS05631_RS25865	1371006..1371743	-	738	WP_050988190.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..1501701	1501701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15471.64 Da
• Isoelectric Point: 8.1045

>T80441 WP_037415845.1 NZ_CP023064:1367134-1367577 [Sinorhizobium sp. CCBAU 05631]
VTFLLDVNVVIALIDPGHVAHDDAHEWFASNGQTAWATCPTTENGVIRIVGSPKYPNSPGSPSLVMEVVRKLRSLPGHSF
WPDDVSLVGSGDIAPAKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTGGNSALHLITTKRS

Nucleotide

• Length: 444 bp

>T80441 NZ_CP023064:1367134-1367577 [Sinorhizobium sp. CCBAU 05631]
GTGACTTTCCTGCTCGACGTCAATGTGGTGATCGCCCTAATCGATCCGGGTCACGTAGCGCATGATGACGCGCACGAGTG
GTTCGCGTCCAATGGACAGACCGCGTGGGCCACTTGCCCCACTACCGAAAATGGTGTCATCCGGATCGTCGGAAGCCCCA
AATATCCCAATTCTCCCGGTTCGCCGTCACTCGTGATGGAGGTAGTCCGAAAATTGCGGTCGCTCCCCGGTCATAGCTTT
TGGCCGGATGATGTGAGCCTTGTCGGGTCAGGTGATATCGCTCCCGCGAAGATCCTGACTTCGGGGCAGGTAACTGACAC
CTACCTACTTGCCCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTATCAGCAGCGGCGGTCACAG
GGGGCAATTCCGCCTTGCACCTGATCACTACAAAGAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8167.45 Da
• Isoelectric Point: 11.5657

>AT80441 WP_037415848.1 NZ_CP023064:1366910-1367137 [Sinorhizobium sp. CCBAU 05631]
MRTTLAIDDDVLIAAKAMAAQQRRSVGEVISELARRSLRRPRGGGERNGIPLLSPRPNAPPVTLEIVNALRDELP

Nucleotide

• Length: 228 bp

>AT80441 NZ_CP023064:1366910-1367137 [Sinorhizobium sp. CCBAU 05631]
ATGAGAACGACCCTGGCGATCGACGATGATGTTTTGATCGCAGCGAAGGCGATGGCAGCCCAACAGCGTCGAAGCGTCGG
CGAGGTCATTTCCGAGCTGGCGCGGCGTTCTCTGCGCCGGCCGCGCGGTGGCGGCGAGCGCAATGGAATTCCTCTCCTGT
CACCCCGGCCGAATGCGCCACCGGTGACGCTCGAAATCGTCAACGCCTTGCGTGACGAACTGCCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A6M9B6