Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 37773..38298 Replicon plasmid pFORC69
Accession NZ_CP023062
Organism Escherichia coli strain FORC_069

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FORC69_RS27500 Protein ID WP_001159871.1
Coordinates 37993..38298 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FORC69_RS27495 Protein ID WP_000813634.1
Coordinates 37773..37991 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC69_RS27470 33548..33973 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
FORC69_RS27475 33970..34320 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
FORC69_RS27480 34351..35775 + 1425 Protein_51 IS66-like element ISEc23 family transposase -
FORC69_RS27485 36029..37162 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
FORC69_RS27490 37223..37411 + 189 Protein_53 IS66 family transposase -
FORC69_RS27495 37773..37991 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC69_RS27500 37993..38298 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC69_RS27505 38299..39108 + 810 WP_000016979.1 site-specific integrase -
FORC69_RS27510 39246..39521 - 276 WP_000239529.1 hypothetical protein -
FORC69_RS27515 39515..40159 - 645 WP_000633911.1 AAA family ATPase -
FORC69_RS27520 40388..41359 + 972 WP_001103689.1 hypothetical protein -
FORC69_RS27525 41364..41756 + 393 WP_000340832.1 plasmid partitioning/stability family protein -
FORC69_RS27530 41788..42615 - 828 Protein_61 translesion error-prone DNA polymerase V subunit UmuC -
FORC69_RS27535 42626..43099 - 474 Protein_62 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B agg3D / agg3C / agg3B / agg3A / aap/aspU / pic 1..77306 77306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T80439 WP_001159871.1 NZ_CP023062:37993-38298 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T80439 NZ_CP023062:37993-38298 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT80439 WP_000813634.1 NZ_CP023062:37773-37991 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT80439 NZ_CP023062:37773-37991 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References